The impact of culture conditions on early follicle recruitment and growth from human ovarian cortex biopsies in vitro

• Verfasst von: Bender-Liebenthron, Jana [VerfasserIn]
• Verfasst von: Köster, Maria [VerfasserIn]
• Verfasst von: Drengner, Christina [VerfasserIn]
• Verfasst von: Reinsberg, Jochen [VerfasserIn]
• Verfasst von: van der Ven, Hans [VerfasserIn]
• Verfasst von: Montag, Markus [VerfasserIn]
• Titel: The impact of culture conditions on early follicle recruitment and growth from human ovarian cortex biopsies in vitro
• Verf.angabe: Jana Liebenthron, M.Sc., Maria Köster, D.V.M., Christina Drengner, Jochen Reinsberg, Ph.D., Hans van der Ven, M.D., and Markus Montag, Ph.D.
• E-Jahr: 2013
• Jahr: April 28, 2013
• Umfang: 14 S.
• Fussnoten: Gesehen am 02.03.2022
• Titel Quelle: Enthalten in: Fertility and sterility
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1950
• Jahr Quelle: 2013
• Band/Heft Quelle: 100(2013), 2 vom: Aug., Seite 483-491.e5
• ISSN Quelle: 1556-5653
• DOI: doi:10.1016/j.fertnstert.2013.03.046
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: early folliculogenesis
• Sach-SW: fertility preservation
• Sach-SW: in vitro fluid culture
• Sach-SW: oncofertility
• Sach-SW: Ovarian cortical tissue
• K10plus-PPN: 1794372199

Abstract

Objective - To investigate the effects of a dynamic fluidic culture system on early in vitro folliculogenesis in standardized ovarian cortex biopsies. - Design - Cortical small strips were cultured for 6 days in a conventional static or in a dynamic fluidic culture system. - Setting - University-affiliated laboratory with an associated cryobank facility. - Patient(s) - Ovarian cortex from postpuberal female cancer patients (26.1 ± 1.3 y) who opted for cryopreservation of their tissue for fertility protection before gonadotoxic cancer therapy. With informed consent of the Institutional Ethics Committee, part of the tissue was available for patient-related research studies. - Intervention(s) - None. - Main Outcome Measure(s) - The viability and proliferative capacity of the cortex biopsies were evaluated by chemiluminescent microparticle immunoassay for detection of in vitro produced E2 and P in the supernate, by viable follicle counting via calcein staining, by histologic analyses, and by total RNA preparation and reverse transcription for real-time polymerase chain reaction of selected early folliculogenesis genes. - Result(s) - The data support the notion that early follicle development can be better achieved in vitro in a dynamic fluidic culture system. The findings are based on the presence of more viable follicles, higher expression levels of early folliculogenesis genes KIT-L, INHB, and GDF9, and the absence of premature luteinization of follicles. - Conclusion(s) - This study provides evidence that dynamic fluidic culture is a promising approach for investigating early follicular recruitment and growth in cortical biopsies. It may serve as a first step in a multistep culture system to design a complex in vitro system for complete folliculogenesis.