Status: Bibliographieeintrag
Standort: ---
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| Online-Ressource |
Verfasst von: | Jahnke, Kevin [VerfasserIn]  |
| Grubmüller, Helmut [VerfasserIn]  |
| Igaev, Maxim [VerfasserIn]  |
| Göpfrich, Kerstin [VerfasserIn]  |
Titel: | Choice of fluorophore affects dynamic DNA nanostructures |
Verf.angabe: | Kevin Jahnke, Helmut Grubmüller, Maxim Igaev and Kerstin Göpfrich |
E-Jahr: | 2021 |
Jahr: | 30 March 2021 |
Umfang: | 10 S. |
Fussnoten: | Gesehen am 03.03.2022 |
Titel Quelle: | Enthalten in: Nucleic acids research |
Ort Quelle: | Oxford : Oxford Univ. Press, 1974 |
Jahr Quelle: | 2021 |
Band/Heft Quelle: | 49(2021), 7 vom: 19. Apr., Seite 4186-4195 |
ISSN Quelle: | 1362-4962 |
Abstract: | The ability to dynamically remodel DNA origami structures or functional nanodevices is highly desired in the field of DNA nanotechnology. Concomitantly, the use of fluorophores to track and validate the dynamics of such DNA-based architectures is commonplace and often unavoidable. It is therefore crucial to be aware of the side effects of popular fluorophores, which are often exchanged without considering the potential impact on the system. Here, we show that the choice of fluorophore can strongly affect the reconfiguration of DNA nanostructures. To this end, we encapsulate a triple-stranded DNA (tsDNA) into water-in-oil compartments and functionalize their periphery with a single-stranded DNA handle (ssDNA). Thus, the tsDNA can bind and unbind from the periphery by reversible opening of the triplex and subsequent strand displacement. Using a combination of experiments, molecular dynamics (MD) simulations, and reaction-diffusion modelling, we demonstrate for 12 different fluorophore combinations that it is possible to alter or even inhibit the DNA nanostructure formation—without changing the DNA sequence. Besides its immediate importance for the design of pH-responsive switches and fluorophore labelling, our work presents a strategy to precisely tune the energy landscape of dynamic DNA nanodevices. |
DOI: | doi:10.1093/nar/gkab201 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1093/nar/gkab201 |
| DOI: https://doi.org/10.1093/nar/gkab201 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1794515968 |
Verknüpfungen: | → Zeitschrift |
Choice of fluorophore affects dynamic DNA nanostructures / Jahnke, Kevin [VerfasserIn]; 30 March 2021 (Online-Ressource)
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