Micelle delivery of doxorubicin increases cytotoxicity to prostate carcinoma cells

• Verfasst von: McNealy, Tamara Lyn [VerfasserIn]
• Verfasst von: Trojan, Lutz [VerfasserIn]
• Verfasst von: Knoll, Thomas [VerfasserIn]
• Verfasst von: Alken, Peter [VerfasserIn]
• Verfasst von: Michel, Maurice Stephan [VerfasserIn]
• Titel: Micelle delivery of doxorubicin increases cytotoxicity to prostate carcinoma cells
• Verf.angabe: Tamara L. McNealy, Lutz Trojan, Thomas Knoll, Peter Alken, Maurice Stephan Michel
• E-Jahr: 2004
• Jahr: 9 January 2004
• Umfang: 6 S.
• Fussnoten: Gesehen am 07.03.2022
• Titel Quelle: Enthalten in: Urological research
• Ort Quelle: Berlin : Springer, 1973
• Jahr Quelle: 2004
• Band/Heft Quelle: 32(2004), 4, Seite 255-260
• ISSN Quelle: 1434-0879
• DOI: doi:10.1007/s00240-003-0321-6
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1794925651

Abstract

The use of doxorubicin as a chemotherapeutic agent is hindered by its toxic side effects on the normal cells of the body. The objective of this study was to determine if micelle-delivered doxorubicin could increase the effectiveness of doxorubicin against prostate carcinoma cells. Rat prostate carcinoma cells (MatLu) were cultured under standard conditions. Phosphate-buffered saline (PBS), doxorubicin and/or micelle solution (Pluronic 10500 solution) was added to the cell suspensions and incubated for 3 h. After incubation, cells were washed twice. Analysis consisted of: 1) immediate cell count and 2) proliferation assay at 24 and 144 h. After 24 h, samples with micelle-incorporated doxorubicin had 75% (10% pluronic with 10 µg/ml doxorubicin) and 80% (1% pluronic with 10 µg/ml doxorubicin) cell proliferation results compared with the control group. After 144-h incubation, these same two groups demonstrated cell proliferation results of only 30 and 43% of the control group. The in vitro cytotoxicity of doxorubicin against prostate carcinoma cells was dramatically increased by incorporating the molecule with polymeric micelles.