Coatomer and dimeric ADP ribosylation factor 1 promote distinct steps in membrane scission

• Verfasst von: Beck, Rainer [VerfasserIn]
• Verfasst von: Prinz, Simone [VerfasserIn]
• Verfasst von: Diestelkötter-Bachert, Petra [VerfasserIn]
• Verfasst von: Röhling, Simone [VerfasserIn]
• Verfasst von: Adolf, Frank [VerfasserIn]
• Verfasst von: Höhner, Kathrin [VerfasserIn]
• Verfasst von: Welsch, Sonja [VerfasserIn]
• Verfasst von: Ronchi, Paolo [VerfasserIn]
• Verfasst von: Brügger, Britta [VerfasserIn]
• Verfasst von: Briggs, John A. G. [VerfasserIn]
• Verfasst von: Wieland, Felix T. [VerfasserIn]
• Titel: Coatomer and dimeric ADP ribosylation factor 1 promote distinct steps in membrane scission
• Verf.angabe: Rainer Beck, Simone Prinz, Petra Diestelkötter-Bachert, Simone Röhling, Frank Adolf, Kathrin Hoehner, Sonja Welsch, Paolo Ronchi, Britta Brügger, John A.G. Briggs, and Felix Wieland
• E-Jahr: 2011
• Jahr: September 05 2011
• Umfang: 13 S.
• Fussnoten: Gesehen am 10.03.2022
• Titel Quelle: Enthalten in: The journal of cell biology
• Ort Quelle: New York, NY : Rockefeller Univ. Press, 1962
• Jahr Quelle: 2011
• Band/Heft Quelle: 194(2011), 5, Seite 765-777
• ISSN Quelle: 1540-8140
• DOI: doi:10.1083/jcb.201011027
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 179523511X

Abstract

Formation of coated vesicles requires two striking manipulations of the lipid bilayer. First, membrane curvature is induced to drive bud formation. Second, a scission reaction at the bud neck releases the vesicle. Using a reconstituted system for COPI vesicle formation from purified components, we find that a dimerization-deficient Arf1 mutant, which does not display the ability to modulate membrane curvature in vitro or to drive formation of coated vesicles, is able to recruit coatomer to allow formation of COPI-coated buds but does not support scission. Chemical cross-linking of this Arf1 mutant restores vesicle release. These experiments show that initial curvature of the bud is defined primarily by coatomer, whereas the membrane curvature modulating activity of dimeric Arf1 is required for membrane scission.