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| Verfasst von: | Banterle, Niccolò [VerfasserIn]  |
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| | Nievergelt, Adrian P. [VerfasserIn] |
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| | Buhr, Svenja de [VerfasserIn] |
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| | Hatzopoulos, Georgios N. [VerfasserIn] |
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| | Brillard, Charlène [VerfasserIn] |
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| | Andany, Santiago [VerfasserIn] |
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| | Hübscher, Tania [VerfasserIn] |
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| | Sorgenfrei, Frieda A. [VerfasserIn] |
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| | Schwarz, Ulrich S. [VerfasserIn] |
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| | Gräter, Frauke [VerfasserIn] |
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| | Fantner, Georg E. [VerfasserIn] |
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| | Gönczy, Pierre [VerfasserIn] |
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| Titel: | Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture |
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| Verf.angabe: | Niccolò Banterle, Adrian P. Nievergelt, Svenja de Buhr, Georgios N. Hatzopoulos, Charlène Brillard, Santiago Andany, Tania Hübscher, Frieda A. Sorgenfrei, Ulrich S. Schwarz, Frauke Gräter, Georg E. Fantner & Pierre Gönczy |
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| E-Jahr: | 2021 |
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| Jahr: | 26 October 2021 |
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| Umfang: | 14 S. |
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| Fussnoten: | Gesehen am 10.03.2022 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2010 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 12(2021), Artikel-ID 6180, Seite 1-14 |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | Discovering mechanisms governing organelle assembly is a fundamental pursuit in biology. The centriole is an evolutionarily conserved organelle with a signature 9-fold symmetrical chiral arrangement of microtubules imparted onto the cilium it templates. The first structure in nascent centrioles is a cartwheel, which comprises stacked 9-fold symmetrical SAS-6 ring polymers emerging orthogonal to a surface surrounding each resident centriole. The mechanisms through which SAS-6 polymerization ensures centriole organelle architecture remain elusive. We deploy photothermally-actuated off-resonance tapping high-speed atomic force microscopy to decipher surface SAS-6 self-assembly mechanisms. We show that the surface shifts the reaction equilibrium by ~104 compared to solution. Moreover, coarse-grained molecular dynamics and atomic force microscopy reveal that the surface converts the inherent helical propensity of SAS-6 polymers into 9-fold rings with residual asymmetry, which may guide ring stacking and impart chiral features to centrioles and cilia. Overall, our work reveals fundamental design principles governing centriole assembly. |
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| DOI: | doi:10.1038/s41467-021-26329-1 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext ; Verlag: https://doi.org/10.1038/s41467-021-26329-1 |
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| | kostenfrei: Volltext: https://www.nature.com/articles/s41467-021-26329-1 |
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| | DOI: https://doi.org/10.1038/s41467-021-26329-1 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Bibliogr. Hinweis: | Forschungsdaten: Buhr, Svenja de: Dual kinetic and structural role for the surface in guiding SAS-6 self-assembly to direct centriole architecture [data] |
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| Sach-SW: | Organelles |
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| | Proteins |
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| | Supramolecular assembly |
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| K10plus-PPN: | 179531446X |
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| Verknüpfungen: | → Zeitschrift |
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Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture / Banterle, Niccolò [VerfasserIn]; 26 October 2021 (Online-Ressource)
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