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Verfasst von:Gößler, Ulrich [VerfasserIn]   i
 Bieback, Karen [VerfasserIn]   i
 Bugert, Peter [VerfasserIn]   i
 Heller, Tobias Christian [VerfasserIn]   i
 Sadick, Haneen [VerfasserIn]   i
 Hörmann, Karl [VerfasserIn]   i
 Riedel, Frank [VerfasserIn]   i
Titel:In vitro analysis of integrin expression during chondrogenic differentiation of mesenchymal stem cells and chondrocytes upon dedifferentiation in cell culture
Verf.angabe:Ulrich Reinhart Goessler, Karen Bieback, Peter Bugert, Tobias Heller, Haneen Sadick, Karl Hörmann and Frank Riedel
E-Jahr:2006
Jahr:February 1, 2006
Umfang:7 S.
Fussnoten:Gesehen am 11.03.2022
Titel Quelle:Enthalten in: International journal of molecular medicine
Ort Quelle:Athens : Spandidos Publ., 1998
Jahr Quelle:2006
Band/Heft Quelle:17(2006), 2, Seite 301-307
ISSN Quelle:1791-244X
Abstract:Tissue engineering represents a promising method for generating chondrogenic grafts for reconstructive surgery. In cultured chondrocytes, the dedifferentiation of cells seems unavoidable for multiplication. Stem cells, however, displaying unlimited self-renewal and the capacity to differentiate towards chondrocytes, might be usable after further characterization. As the interactions between the extracellular matrix and the cellular compartment can alter the cellular behaviour, we investigated the expression of integrins using microarray analysis during chondrogenic differentiation of human mesenchymal stem cells (MSC) in comparison with dedifferentiatiating human chondrocytes (HC) harvested during septoplasty. During chondrogenic differentiation of MSC, the fibronectin-receptor (Integrin β1α5), fibronectin and the GPIIb/IIIa-receptor were downregulated. The components of the vitronectin-receptor (Integrin αvβ3) and CD47 were constantly expressed and ILK was downregulated. Vitronectin and osteopontin were not expressed by the cells. In HC, Integrin β1α5 in conjunction with the ligand fibronectin were upregulated during dedifferentiation, Integrin αvβ3 as well as the GBIIb/IIIa-receptor were activated on day 21 but neither vitronectin nor osteopontin were expressed by the cells. The integrins, β2, β4, β6, β8 and α2, α4, α6, α7, α11, were not expressed at any time. ILK, CD47, and ICAP were activated with ongoing dedifferentiation. In conclusion, a candidate for signal-transmission is the fibronectin receptor (integrin α5β1) in conjunction with its ligand fibronectin. Other receptors, e.g. for vitronectin and osteopontin (αvβ3), or their ligands do not seem to be involved in signal transmission for dedifferentiation. The GPIIb/IIIa-receptor might assist the process of dedifferentiation. Intracellularly, ILK, ICAP1 and CD47 might be involved in the transduction of integrin-dependent signals.
DOI:doi:10.3892/ijmm.17.2.301
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.3892/ijmm.17.2.301
 Volltext: https://www.spandidos-publications.com/10.3892/ijmm.17.2.301
 DOI: https://doi.org/10.3892/ijmm.17.2.301
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1795467452
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