| |  Online-Ressource |
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| Verfasst von: | Reichman, Avinoam [VerfasserIn]  |
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| | Kunz, Alexander [VerfasserIn] |
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| | Joedicke, Jara Johanna [VerfasserIn] |
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| | Höpken, Uta [VerfasserIn] |
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| | Keib, Anna [VerfasserIn] |
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| | Neuber, Brigitte [VerfasserIn] |
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| | Sedloev, David [VerfasserIn] |
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| | Wang, Lei [VerfasserIn] |
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| | Jiang, Genqiao [VerfasserIn] |
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| | Hückelhoven-Krauss, Angela [VerfasserIn] |
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| | Eberhardt, Franziska [VerfasserIn] |
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| | Müller-Tidow, Carsten [VerfasserIn] |
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| | Wermke, Martin [VerfasserIn] |
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| | Rehm, Armin [VerfasserIn] |
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| | Schmitt, Michael [VerfasserIn] |
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| | Schmitt, Anita [VerfasserIn] |
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| Titel: | Comparison of FACS and PCR for detection of BCMA-CAR-T cells |
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| Verf.angabe: | Avinoam Reichman, Alexander Kunz, Jara J. Joedicke, Uta E. Höpken, Anna Keib, Brigitte Neuber, David Sedloev, Lei Wang, Genqiao Jiang, Angela Hückelhoven-Krauss, Franziska Eberhardt, Carsten Müller-Tidow, Martin Wermke, Armin Rehm, Michael Schmitt and Anita Schmitt |
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| E-Jahr: | 2022 |
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| Jahr: | 14 January 2022 |
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| Umfang: | 14 S. |
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| Fussnoten: | Gesehen am 22.03.2022 |
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| Titel Quelle: | Enthalten in: International journal of molecular sciences |
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| Ort Quelle: | Basel : Molecular Diversity Preservation International, 2000 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 23(2022), 2, Artikel-ID 903, Seite 1-14 |
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| ISSN Quelle: | 1422-0067 |
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| | 1661-6596 |
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| Abstract: | Chimeric-antigen-receptor (CAR)-T-cell therapy is already widely used to treat patients who are relapsed or refractory to chemotherapy, antibodies, or stem-cell transplantation. Multiple myeloma still constitutes an incurable disease. CAR-T-cell therapy that targets BCMA (B-cell maturation antigen) is currently revolutionizing the treatment of those patients. To monitor and improve treatment outcomes, methods to detect CAR-T cells in human peripheral blood are highly desirable. In this study, three different detection reagents for staining BCMA-CAR-T cells by flow cytometry were compared. Moreover, a quantitative polymerase chain reaction (qPCR) to detect BCMA-CAR-T cells was established. By applying a cell-titration experiment of BCMA-CAR-T cells, both methods were compared head-to-head. In flow-cytometric analysis, the detection reagents used in this study could all detect BCMA-CAR-T cells at a similar level. The results of false-positive background staining differed as follows (standard deviation): the BCMA-detection reagent used on the control revealed a background staining of 0.04% (±0.02%), for the PE-labeled human BCMA peptide it was 0.25% (±0.06%) and for the polyclonal anti-human IgG antibody it was 7.2% (±9.2%). The ability to detect BCMA-CAR-T cells down to a concentration of 0.4% was similar for qPCR and flow cytometry. The qPCR could detect even lower concentrations (0.02-0.01%). In summary, BCMA-CAR-T-cell monitoring can be reliably performed by both flow cytometry and qPCR. In flow cytometry, reagents with low background staining should be preferred. |
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| DOI: | doi:10.3390/ijms23020903 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext ; Verlag: https://doi.org/10.3390/ijms23020903 |
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| | kostenfrei: Volltext: https://www.mdpi.com/1422-0067/23/2/903 |
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| | DOI: https://doi.org/10.3390/ijms23020903 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | BCMA-CAR |
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| | detection reagent |
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| | flow cytometry |
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| | polymerase chain reaction |
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| K10plus-PPN: | 1796018821 |
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| Verknüpfungen: | → Zeitschrift |
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Comparison of FACS and PCR for detection of BCMA-CAR-T cells / Reichman, Avinoam [VerfasserIn]; 14 January 2022 (Online-Ressource)
