| Online-Ressource |
Verfasst von: | Archer, Stuart K. [VerfasserIn]  |
| Inchaustegui Gil, Diana Patricia [VerfasserIn]  |
| Queiroz, Rafael [VerfasserIn]  |
| Clayton, Christine [VerfasserIn]  |
Titel: | The cell cycle regulated transcriptome of Trypanosoma brucei |
Verf.angabe: | Stuart K. Archer, Diana Inchaustegui, Rafael Queiroz, Christine Clayton |
E-Jahr: | 2011 |
Jahr: | March 31, 2011 |
Umfang: | 14 S. |
Fussnoten: | Gesehen am 18.03.2022 |
Titel Quelle: | Enthalten in: PLOS ONE |
Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
Jahr Quelle: | 2011 |
Band/Heft Quelle: | 6(2011), 3, Artikel-ID e18425, Seite 1-14 |
ISSN Quelle: | 1932-6203 |
Abstract: | Progression of the eukaryotic cell cycle requires the regulation of hundreds of genes to ensure that they are expressed at the required times. Integral to cell cycle progression in yeast and animal cells are temporally controlled, progressive waves of transcription mediated by cell cycle-regulated transcription factors. However, in the kinetoplastids, a group of early-branching eukaryotes including many important pathogens, transcriptional regulation is almost completely absent, raising questions about the extent of cell-cycle regulation in these organisms and the mechanisms whereby regulation is achieved. Here, we analyse gene expression over the Trypanosoma brucei cell cycle, measuring changes in mRNA abundance on a transcriptome-wide scale. We developed a “double-cut” elutriation procedure to select unperturbed, highly synchronous cell populations from log-phase cultures, and compared this to synchronization by starvation. Transcriptome profiling over the cell cycle revealed the regulation of at least 430 genes. While only a minority were homologous to known cell cycle regulated transcripts in yeast or human, their functions correlated with the cellular processes occurring at the time of peak expression. We searched for potential target sites of RNA-binding proteins in these transcripts, which might earmark them for selective degradation or stabilization. Over-represented sequence motifs were found in several co-regulated transcript groups and were conserved in other kinetoplastids. Furthermore, we found evidence for cell-cycle regulation of a flagellar protein regulon with a highly conserved sequence motif, bearing similarity to consensus PUF-protein binding motifs. RNA sequence motifs that are functional in cell-cycle regulation were more widespread than previously expected and conserved within kinetoplastids. These findings highlight the central importance of post-transcriptional regulation in the proliferation of parasitic kinetoplastids. |
DOI: | doi:10.1371/journal.pone.0018425 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0018425 |
| Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0018425 |
| DOI: https://doi.org/10.1371/journal.pone.0018425 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Cell cycle and cell division |
| Gene expression |
| Gene regulation |
| Kinetoplastids |
| Post-transcriptional gene regulation |
| Sequence motif analysis |
| Synthesis phase |
| Trypanosoma brucei gambiense |
K10plus-PPN: | 1796047678 |
Verknüpfungen: | → Zeitschrift |
¬The¬ cell cycle regulated transcriptome of Trypanosoma brucei / Archer, Stuart K. [VerfasserIn]; March 31, 2011 (Online-Ressource)