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| Verfasst von: | Werther, Philipp [VerfasserIn]  |
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| | Yserentant, Klaus [VerfasserIn] |
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| | Braun, Felix [VerfasserIn] |
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| | Grußmayer, Kristin Stefanie [VerfasserIn] |
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| | Navikas, Vytautas [VerfasserIn] |
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| | Yu, Miao [VerfasserIn] |
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| | Zhang, Zhibin [VerfasserIn] |
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| | Ziegler, Michael J. [VerfasserIn] |
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| | Mayer, Christoph [VerfasserIn] |
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| | Gralak, Antoni J. [VerfasserIn] |
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| | Busch, Marvin [VerfasserIn] |
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| | Chi, Weijie [VerfasserIn] |
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| | Rominger, Frank [VerfasserIn] |
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| | Radenovic, Aleksandra [VerfasserIn] |
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| | Liu, Xiaogang [VerfasserIn] |
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| | Lemke, Edward A. [VerfasserIn] |
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| | Buckup, Tiago [VerfasserIn] |
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| | Herten, Dirk-Peter [VerfasserIn] |
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| | Wombacher, Richard [VerfasserIn] |
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| Titel: | Bio-orthogonal red and far-red fluorogenic probes for wash-free live-cell and super-resolution microscopy |
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| Verf.angabe: | Philipp Werther, Klaus Yserentant, Felix Braun, Kristin Grußmayer, Vytautas Navikas, Miao Yu, Zhibin Zhang, Michael J. Ziegler, Christoph Mayer, Antoni J. Gralak, Marvin Busch, Weijie Chi, Frank Rominger, Aleksandra Radenovic, Xiaogang Liu, Edward A. Lemke, Tiago Buckup, Dirk-Peter Herten, and Richard Wombacher |
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| E-Jahr: | 2021 |
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| Jahr: | August 20, 2021 |
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| Umfang: | 11 S. |
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| Fussnoten: | Published: August 20, 2021 ; Gesehen am 23.03.2022 |
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| Titel Quelle: | Enthalten in: American Chemical SocietyACS central science |
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| Ort Quelle: | Washington, DC : ACS Publ., 2015 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 7(2021), 9, Seite 1561-1571 |
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| ISSN Quelle: | 2374-7951 |
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| Abstract: | Small-molecule fluorophores enable the observation of biomolecules in their native context with fluorescence microscopy. Specific labeling via bio-orthogonal tetrazine chemistry combines minimal label size with rapid labeling kinetics. At the same time, fluorogenic tetrazine-dye conjugates exhibit efficient quenching of dyes prior to target binding. However, live-cell compatible long-wavelength fluorophores with strong fluorogenicity have been difficult to realize. Here, we report close proximity tetrazine-dye conjugates with minimal distance between tetrazine and the fluorophore. Two synthetic routes give access to a series of cell-permeable and -impermeable dyes including highly fluorogenic far-red emitting derivatives with electron exchange as the dominant excited-state quenching mechanism. We demonstrate their potential for live-cell imaging in combination with unnatural amino acids, wash-free multicolor and super-resolution STED, and SOFI imaging. These dyes pave the way for advanced fluorescence imaging of biomolecules with minimal label size. |
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| DOI: | doi:10.1021/acscentsci.1c00703 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: https://doi.org/10.1021/acscentsci.1c00703 |
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| | DOI: https://doi.org/10.1021/acscentsci.1c00703 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1796386863 |
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| Verknüpfungen: | → Zeitschrift |
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Bio-orthogonal red and far-red fluorogenic probes for wash-free live-cell and super-resolution microscopy / Werther, Philipp [VerfasserIn]; August 20, 2021 (Online-Ressource)
