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| Verfasst von: | Biffi, Alessandra [VerfasserIn]  |
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| | Bartholomä, Cynthia C. [VerfasserIn] |
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| | Cesana, Daniela [VerfasserIn] |
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| | Cartier, Natalie [VerfasserIn] |
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| | Aubourg, Patrik [VerfasserIn] |
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| | Ranzani, Marco [VerfasserIn] |
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| | Cesani, Martina [VerfasserIn] |
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| | Benedicenti, Fabrizio [VerfasserIn] |
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| | Plati, Tiziana [VerfasserIn] |
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| | Rubagotti, Enrico [VerfasserIn] |
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| | Merella, Stefania [VerfasserIn] |
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| | Capotondo, Alessia [VerfasserIn] |
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| | Sgualdino, Jacopo [VerfasserIn] |
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| | Zanetti, Gianluigi [VerfasserIn] |
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| | Kalle, Christof von [VerfasserIn] |
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| | Schmidt, Manfred [VerfasserIn] |
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| | Naldini, Luigi [VerfasserIn] |
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| | Montini, Eugenio [VerfasserIn] |
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| Titel: | Lentiviral vector common integration sites in preclinical models and a clinical trial reflect a benign integration bias and not oncogenic selection |
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| Verf.angabe: | Alessandra Biffi, Cynthia C. Bartolomae, Daniela Cesana, Natalie Cartier, Patrik Aubourg, Marco Ranzani, Martina Cesani, Fabrizio Benedicenti, Tiziana Plati, Enrico Rubagotti, Stefania Merella, Alessia Capotondo, Jacopo Sgualdino, Gianluigi Zanetti, Christof von Kalle, Manfred Schmidt, Luigi Naldini, and Eugenio Montini |
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| E-Jahr: | 2011 |
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| Jahr: | May 19 2011 |
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| Umfang: | 8 S. |
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| Fussnoten: | Gesehen am 23.03.2022 |
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| Titel Quelle: | Enthalten in: Blood |
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| Ort Quelle: | Washington, DC : American Society of Hematology, 1946 |
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| Jahr Quelle: | 2011 |
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| Band/Heft Quelle: | 117(2011), 20, Seite 5332-5339 |
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| ISSN Quelle: | 1528-0020 |
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| Abstract: | A recent clinical trial for adrenoleukodystrophy (ALD) showed the efficacy and safety of lentiviral vector (LV) gene transfer in hematopoietic stem progenitor cells. However, several common insertion sites (CIS) were found in patients' cells, suggesting that LV integrations conferred a selective advantage. We performed high-throughput LV integration site analysis on human hematopoietic stem progenitor cells engrafted in immunodeficient mice and found the same CISs reported in patients with ALD. Strikingly, most CISs in our experimental model and in patients with ALD cluster in megabase-wide chromosomal regions of high LV integration density. Conversely, cancer-triggering integrations at CISs found in tumor cells from γretroviral vector-based clinical trials and oncogene-tagging screenings in mice always target a single gene and are contained in narrow genomic intervals. These findings imply that LV CISs are produced by an integration bias toward specific genomic regions rather than by oncogenic selection. |
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| DOI: | doi:10.1182/blood-2010-09-306761 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1182/blood-2010-09-306761 |
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| | DOI: https://doi.org/10.1182/blood-2010-09-306761 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1796409367 |
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| Verknüpfungen: | → Zeitschrift |
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Lentiviral vector common integration sites in preclinical models and a clinical trial reflect a benign integration bias and not oncogenic selection / Biffi, Alessandra [VerfasserIn]; May 19 2011 (Online-Ressource)
