Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Bikou, Olympia [VerfasserIn]   i
 Thomas, Dierk [VerfasserIn]   i
 Trappe, Kerstin [VerfasserIn]   i
 Lugenbiel, Patrick [VerfasserIn]   i
 Kelemen, Kamilla [VerfasserIn]   i
 Koch, Martin Christoph [VerfasserIn]   i
 Soucek, Radim [VerfasserIn]   i
 Voss, Frederik [VerfasserIn]   i
 Becker, Rüdiger [VerfasserIn]   i
 Katus, Hugo [VerfasserIn]   i
 Bauer, Alexander [VerfasserIn]   i
Titel:Connexin 43 gene therapy prevents persistent atrial fibrillation in a porcine model
Verf.angabe:Olympia Bikou, Dierk Thomas, Kerstin Trappe, Patrick Lugenbiel, Kamilla Kelemen, Martin Koch, Radim Soucek, Frederik Voss, Rüdiger Becker, Hugo A. Katus, and Alexander Bauer
E-Jahr:2011
Jahr:28 July 2011
Umfang:8 S.
Fussnoten:Gesehen am 23.03.2022
Titel Quelle:Enthalten in: Cardiovascular research
Ort Quelle:Oxford : Oxford University Press, 1967
Jahr Quelle:2011
Band/Heft Quelle:92(2011), 2, Seite 218-225
ISSN Quelle:1755-3245
Abstract:Atrial fibrillation (AF) is the most common sustained cardiac arrhythmia, and effective treatment of AF still remains an unmet medical need. AF is associated with atrial conduction disturbances caused by electrical and/or structural remodelling. We hypothesized that AF suppresses expression of the gap junction protein connexin (Cx) 43 and that Cx43 gene transfer to both atria would prevent persistent AF. The first aim of this study was to assess whether AF is associated with connexin remodelling in a porcine model. A strategy to suppress persistent AF by gene therapy was then developed and evaluated in vivo.AF was induced in domestic pigs via atrial burst pacing, causing a 62.4% reduction in atrial Cx43 protein. Adenoviruses encoding for Cx43 (AdCx43) or green fluorescent protein (AdGFP) were injected into both atria, followed by epicardial electroporation to enhance transgene expression. Combining direct injection of adenoviruses with electroporation achieved GFP reporter gene expression in ∼50% of atrial cells in vivo. AdCx43-treated animals exhibited a 2.5-fold increase in atrial Cx43 protein content and did not develop persistent AF during the observation period of 14 days. In contrast, control animals developed persistent AF within 7.4 ± 0.5 days. Rapid ventricular heart rates during AF led to deterioration of cardiac function in control pigs but not in pigs treated with AdCx43.Our results highlight the contribution of Cx43 to the pathophysiology of AF and demonstrate the viability of gene therapy for prevention of atrial arrhythmias.
DOI:doi:10.1093/cvr/cvr209
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1093/cvr/cvr209
 DOI: https://doi.org/10.1093/cvr/cvr209
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1796411418
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68898781   QR-Code
zum Seitenanfang