| |  Online-Ressource |
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| Verfasst von: | Manigold, Tobias [VerfasserIn]  |
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| | Böcker, Ulrich [VerfasserIn] |
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| | Traber, Petra Sieglinde [VerfasserIn] |
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| | Si, Tuan-Dong [VerfasserIn] |
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| | Kurimoto, Masashi [VerfasserIn] |
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| | Hanck, Christoph [VerfasserIn] |
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| | Singer, Manfred V. [VerfasserIn] |
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| | Rossol, Siegbert [VerfasserIn] |
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| Titel: | Lipopolysaccharide/endotoxin induces IL-18 via CD14 in human peripheral blood mononuclear cells in vitro |
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| Verf.angabe: | Tobias Manigold, Ulrich Böcker, Petra Traber, Tuan Dong-Si, Masashi Kurimoto, Christoph Hanck, Manfred V. Singer, Siegbert Rossol |
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| Jahr: | 2000 |
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| Umfang: | 5 S. |
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| Fussnoten: | Available online 25 May 2002 ; Gesehen am 25.03.2022 |
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| Titel Quelle: | Enthalten in: Cytokine |
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| Ort Quelle: | Oxford [u.a.] : Elsevier, 1989 |
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| Jahr Quelle: | 2000 |
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| Band/Heft Quelle: | 12(2000), 12 vom: Dez., Seite 1788-1792 |
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| ISSN Quelle: | 1096-0023 |
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| Abstract: | IL-18 shares activities with IL-12 in generating T-helper 1 cells and cytokine response. It mediates LPS/endotoxin lethality by IL-12 independent interferon-γ synthesis and it induces bacteria-related organ failure. As peripheral blood mononuclear cells (PBMC) are potent producers of IL-18, we studied the regulation of IL-18 upon exposure to LPS and Staphylococcus aureus (SAC) in vitro. Freshly isolated PBMC constitutively expressed IL-18 mRNA. After unstimulated preincubation for 48h, however, IL-18 transcripts were nearly not detectable by RT-PCR, but inducible by LPS or SAC (P<0.01). Both LPS and SAC were potent stimuli of IL-18 protein secretion (P<0.01). LPS-mediated IL-18 gene expression and secretion was CD14-dependent and significantly inhibited by co-incubation of PBMC with neutralizing CD14 antibody (P<0.01). We conclude that LPS-driven IL-18 is dependent on the expression of costimulatory factors and that IL-18 inhibition might attenuate IL-18-related toxic effects. |
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| DOI: | doi:10.1006/cyto.2000.0783 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1006/cyto.2000.0783 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S104346660090783X |
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| | DOI: https://doi.org/10.1006/cyto.2000.0783 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | IL-18/endotoxin/CD14/PBMC |
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| K10plus-PPN: | 179667611X |
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| Verknüpfungen: | → Zeitschrift |
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Lipopolysaccharide/endotoxin induces IL-18 via CD14 in human peripheral blood mononuclear cells in vitro / Manigold, Tobias [VerfasserIn]; 2000 (Online-Ressource)
