Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Schummer, Bernhard [VerfasserIn]  |
| Siegsmund, Michael [VerfasserIn]  |
| Steidler, Annette [VerfasserIn]  |
| Toktomambetova, Lira [VerfasserIn]  |
| Köhrmann, Kai-Uwe [VerfasserIn]  |
| Alken, Peter [VerfasserIn]  |
Titel: | Expression of the gene for the multidrug resistance-associated protein in human prostate tissue |
Verf.angabe: | Bernhard Schummer, Michael Siegsmund, Anette Steidler, Lira Toktomambetova, Kai-Uwe Köhrmann, Peter Alken |
E-Jahr: | 1999 |
Jahr: | June 1999 |
Umfang: | 5 S. |
Fussnoten: | Gesehen am 28.03.2022 |
Titel Quelle: | Enthalten in: Urological research |
Ort Quelle: | Berlin : Springer, 1973 |
Jahr Quelle: | 1999 |
Band/Heft Quelle: | 27(1999), 3, Seite 164-168 |
ISSN Quelle: | 1434-0879 |
Abstract: | To characterize the clinical relevance of MRP gene in the chemoresistance of prostate carcinomas we determined the multidrug resistance-associated protein (MRP) expression in 30 samples from organ-confined prostate carcinoma, 9 samples from adjacent normal tissue and 4 hormone unresponsive cancers. The measurement of MRP expression was carried out by reverse transcription polymerase chain reaction (RT-PCR) in combination with capillary electrophoresis. Incorporated fluorescence-labeled primers were disclosed by a laser-operated fluorescence detection module. MRP expression was quantified by integration of the peak area and correlated to the ubiquitously expressed β2 microglobulin. As positive control served the adriamycin-resistant HL60-ADR cell line, which overexpresses MRP. MRP expression was found in all samples. All samples showed a lower MRP/β2 ratio than HL60-ADR cells. The expression of the MRP gene was 30% higher in organ-confined tumors than in hormone-unresponsive anaplastic tumors. Normal tissue showed the same MRP mRNA level as the adriamycin-sensitive HL60 cells. A higher tumor stage correlated with an increase of MRP expression (>factor 2), whereas G3 tumors displayed a MRP expression 30% lower than in G2 tumors. The small alterations indicate that MRP expression seems not be involved in the chemoresistance of prostate carcinomas. |
DOI: | doi:10.1007/s002400050104 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1007/s002400050104 |
| DOI: https://doi.org/10.1007/s002400050104 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1796815047 |
Verknüpfungen: | → Zeitschrift |
Expression of the gene for the multidrug resistance-associated protein in human prostate tissue / Schummer, Bernhard [VerfasserIn]; June 1999 (Online-Ressource)
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