Expression of the gene for the multidrug resistance-associated protein in human prostate tissue

• Verfasst von: Schummer, Bernhard [VerfasserIn]
• Verfasst von: Siegsmund, Michael [VerfasserIn]
• Verfasst von: Steidler, Annette [VerfasserIn]
• Verfasst von: Toktomambetova, Lira [VerfasserIn]
• Verfasst von: Köhrmann, Kai-Uwe [VerfasserIn]
• Verfasst von: Alken, Peter [VerfasserIn]
• Titel: Expression of the gene for the multidrug resistance-associated protein in human prostate tissue
• Verf.angabe: Bernhard Schummer, Michael Siegsmund, Anette Steidler, Lira Toktomambetova, Kai-Uwe Köhrmann, Peter Alken
• E-Jahr: 1999
• Jahr: June 1999
• Umfang: 5 S.
• Fussnoten: Gesehen am 28.03.2022
• Titel Quelle: Enthalten in: Urological research
• Ort Quelle: Berlin : Springer, 1973
• Jahr Quelle: 1999
• Band/Heft Quelle: 27(1999), 3, Seite 164-168
• ISSN Quelle: 1434-0879
• DOI: doi:10.1007/s002400050104
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1796815047

Abstract

To characterize the clinical relevance of MRP gene in the chemoresistance of prostate carcinomas we determined the multidrug resistance-associated protein (MRP) expression in 30 samples from organ-confined prostate carcinoma, 9 samples from adjacent normal tissue and 4 hormone unresponsive cancers. The measurement of MRP expression was carried out by reverse transcription polymerase chain reaction (RT-PCR) in combination with capillary electrophoresis. Incorporated fluorescence-labeled primers were disclosed by a laser-operated fluorescence detection module. MRP expression was quantified by integration of the peak area and correlated to the ubiquitously expressed β2 microglobulin. As positive control served the adriamycin-resistant HL60-ADR cell line, which overexpresses MRP. MRP expression was found in all samples. All samples showed a lower MRP/β2 ratio than HL60-ADR cells. The expression of the MRP gene was 30% higher in organ-confined tumors than in hormone-unresponsive anaplastic tumors. Normal tissue showed the same MRP mRNA level as the adriamycin-sensitive HL60 cells. A higher tumor stage correlated with an increase of MRP expression (>factor 2), whereas G3 tumors displayed a MRP expression 30% lower than in G2 tumors. The small alterations indicate that MRP expression seems not be involved in the chemoresistance of prostate carcinomas.