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Verfasst von:Schummer, Bernhard [VerfasserIn]   i
 Siegsmund, Michael [VerfasserIn]   i
 Steidler, Annette [VerfasserIn]   i
 Toktomambetova, Lira [VerfasserIn]   i
 Köhrmann, Kai-Uwe [VerfasserIn]   i
 Alken, Peter [VerfasserIn]   i
Titel:Expression of the gene for the multidrug resistance-associated protein in human prostate tissue
Verf.angabe:Bernhard Schummer, Michael Siegsmund, Anette Steidler, Lira Toktomambetova, Kai-Uwe Köhrmann, Peter Alken
E-Jahr:1999
Jahr:June 1999
Umfang:5 S.
Fussnoten:Gesehen am 28.03.2022
Titel Quelle:Enthalten in: Urological research
Ort Quelle:Berlin : Springer, 1973
Jahr Quelle:1999
Band/Heft Quelle:27(1999), 3, Seite 164-168
ISSN Quelle:1434-0879
Abstract:To characterize the clinical relevance of MRP gene in the chemoresistance of prostate carcinomas we determined the multidrug resistance-associated protein (MRP) expression in 30 samples from organ-confined prostate carcinoma, 9 samples from adjacent normal tissue and 4 hormone unresponsive cancers. The measurement of MRP expression was carried out by reverse transcription polymerase chain reaction (RT-PCR) in combination with capillary electrophoresis. Incorporated fluorescence-labeled primers were disclosed by a laser-operated fluorescence detection module. MRP expression was quantified by integration of the peak area and correlated to the ubiquitously expressed β2 microglobulin. As positive control served the adriamycin-resistant HL60-ADR cell line, which overexpresses MRP. MRP expression was found in all samples. All samples showed a lower MRP/β2 ratio than HL60-ADR cells. The expression of the MRP gene was 30% higher in organ-confined tumors than in hormone-unresponsive anaplastic tumors. Normal tissue showed the same MRP mRNA level as the adriamycin-sensitive HL60 cells. A higher tumor stage correlated with an increase of MRP expression (>factor 2), whereas G3 tumors displayed a MRP expression 30% lower than in G2 tumors. The small alterations indicate that MRP expression seems not be involved in the chemoresistance of prostate carcinomas.
DOI:doi:10.1007/s002400050104
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1007/s002400050104
 DOI: https://doi.org/10.1007/s002400050104
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1796815047
Verknüpfungen:→ Zeitschrift

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