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Status: Bibliographieeintrag

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Verfasst von:Chutiwitoonchai, Nopporn [VerfasserIn]   i
 Hiyoshi, Masateru [VerfasserIn]   i
 Mwimanzi, Philip [VerfasserIn]   i
 Ueno, Takamasa [VerfasserIn]   i
 Adachi, Akio [VerfasserIn]   i
 Ode, Hirotaka [VerfasserIn]   i
 Sato, Hironori [VerfasserIn]   i
 Fackler, Oliver Till [VerfasserIn]   i
 Okada, Seiji [VerfasserIn]   i
 Suzu, Shinya [VerfasserIn]   i
Titel:The identification of a small molecule compound that reduces HIV-1 nef-mediated viral infectivity enhancement
Verf.angabe:Nopporn Chutiwitoonchai, Masateru Hiyoshi, Philip Mwimanzi, Takamasa Ueno, Akio Adachi, Hirotaka Ode, Hironori Sato, Oliver T. Fackler, Seiji Okada, Shinya Suzu
Jahr:2011
Umfang:12 S.
Fussnoten:Gesehen am 29.03.2022
Titel Quelle:Enthalten in: PLOS ONE
Ort Quelle:San Francisco, California, US : PLOS, 2006
Jahr Quelle:2011
Band/Heft Quelle:6(2011), 11, Artikel-ID e27696, Seite 1-12
ISSN Quelle:1932-6203
Abstract:Nef is a multifunctional HIV-1 protein that accelerates progression to AIDS, and enhances the infectivity of progeny viruses through a mechanism that is not yet understood. Here, we show that the small molecule compound 2c reduces Nef-mediated viral infectivity enhancement. When added to viral producer cells, 2c did not affect the efficiency of viral production itself. However, the infectivity of the viruses produced in the presence of 2c was significantly lower than that of control viruses. Importantly, an inhibitory effect was observed with Nef+ wild-type viruses, but not with viruses produced in the absence of Nef or in the presence of proline-rich PxxP motif-disrupted Nef, both of which displayed significantly reduced intrinsic infectivity. Meanwhile, the overexpression of the SH3 domain of the tyrosine kinase Hck, which binds to a PxxP motif in Nef, also reduced viral infectivity. Importantly, 2c inhibited Hck SH3-Nef binding, which was more marked when Nef was pre-incubated with 2c prior to its incubation with Hck, indicating that both Hck SH3 and 2c directly bind to Nef and that their binding sites overlap. These results imply that both 2c and the Hck SH3 domain inhibit the interaction of Nef with an unidentified host protein and thereby reduce Nef-mediated infectivity enhancement. The first inhibitory compound 2c is therefore a valuable chemical probe for revealing the underlying molecular mechanism by which Nef enhances the infectivity of HIV-1.
DOI:doi:10.1371/journal.pone.0027696
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext ; Verlag: https://doi.org/10.1371/journal.pone.0027696
 kostenfrei: Volltext: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0027696
 DOI: https://doi.org/10.1371/journal.pone.0027696
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Glutathione chromatography
 HEK 293 cells
 HIV-1
 Macrophages
 Plasmid construction
 Transfection
 Viral diseases
 Viral replication
K10plus-PPN:1796908762
Verknüpfungen:→ Zeitschrift

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