High energy phosphate transfer by NDPK B/G β γ complexes - an alternative signaling pathway involved in the regulation of basal cAMP production

• Verfasst von: Hippe, Hans-Jörg [VerfasserIn]
• Verfasst von: Wieland, Thomas [VerfasserIn]
• Titel: High energy phosphate transfer by NDPK B/G β γ complexes - an alternative signaling pathway involved in the regulation of basal cAMP production
• Verf.angabe: Hans-Joerg Hippe, Thomas Wieland
• E-Jahr: 2006
• Jahr: 7 September 2006
• Umfang: 6 S.
• Fussnoten: Gesehen am 31.03.2022
• Titel Quelle: Enthalten in: Journal of bioenergetics and biomembranes
• Ort Quelle: Dordrecht [u.a.] : Springer Science + Business Media B.V, 1970
• Jahr Quelle: 2006
• Band/Heft Quelle: 38(2006), 3-4, Seite 197-203
• ISSN Quelle: 1573-6881
• DOI: doi:10.1007/s10863-006-9035-0
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Cyclic AMP
• Sach-SW: Signal Transduction
• Sach-SW: Models, Molecular
• Sach-SW: GTP-Binding Protein beta Subunits
• Sach-SW: GTP-Binding Protein gamma Subunits
• Sach-SW: Guanosine Diphosphate
• Sach-SW: Guanosine Triphosphate
• Sach-SW: Multiprotein Complexes
• Sach-SW: NM23 Nucleoside Diphosphate Kinases
• Sach-SW: Nucleoside-Diphosphate Kinase
• K10plus-PPN: 1797099043

Abstract

The activation of heterotrimeric G proteins induced by G protein coupled receptors (GPCR) is generally believed to occur by a GDP/GTP exchange at the G protein alpha -subunit. Nevertheless, nucleoside diphosphate kinase (NDPK) and the beta-subunit of G proteins (Gbeta) participate in G protein activation by phosphate transfer reactions leading to the formation of GTP from GDP. Recent work elucidated the role of these reactions. Apparently, the NDPK isoform B (NDPK B) forms a complex with Gbetagamma dimers in which NDPK B acts as a histidine kinase phosphorylating Gbeta at His266. Out of this high energetic phosphoamidate bond the phosphate can be transferred specifically onto GDP. The formed GTP binds to the G protein alpha-subunit and thus activates the respective G protein. Evidence is presented, that this process occurs independent of the classical GPCR-induced GTP/GTP exchange und thus contributes, e.g. to the regulation of basal cAMP synthesis in cells.