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| Verfasst von: | Göttig, Stephan [VerfasserIn]  |
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| | Möbest, Dietrich [VerfasserIn] |
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| | Rüster, Brigitte [VerfasserIn] |
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| | Grace, Bithiah [VerfasserIn] |
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| | Winter, Sylvia [VerfasserIn] |
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| | Seifried, Erhard [VerfasserIn] |
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| | Gilles, Jens [VerfasserIn] |
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| | Wieland, Thomas [VerfasserIn] |
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| | Henschler, Reinhard [VerfasserIn] |
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| Titel: | Role of the monomeric GTPase Rho in hematopoietic progenitor cell migration and transplantation |
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| Verf.angabe: | Stephan Göttig, Dietrich Möbest, Brigitte Rüster, Bithiah Grace, Sylvia Winter, Erhard Seifried, Jens Gille, Thomas Wieland and Reinhard Henschler |
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| Jahr: | 2006 |
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| Umfang: | 10 S. |
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| Fussnoten: | First published: 27 December 2005 ; Gesehen am 31.03.2022 |
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| Titel Quelle: | Enthalten in: European journal of immunology |
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| Ort Quelle: | Weinheim : Wiley-VCH, 1971 |
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| Jahr Quelle: | 2006 |
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| Band/Heft Quelle: | 36(2006), 1, Seite 180-189 |
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| ISSN Quelle: | 1521-4141 |
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| Abstract: | To investigate the role of the monomeric guanosine triphosphatase (GTPase) Rho on migration of hematopoietic progenitor cells (HPC), we employed different clostridial toxins which inhibit the Rho family of GTPases. Pretreatment with C2I-C3, a cell-accessible C3 transferase fusion protein that targets Rho, increased chemokinetic migration of the factor-dependent multipotent cell line Factor Dependent Cell Paterson with mixed lineage differentiation potential (FDCP-mix) and of primary lineage marker-depleted HPC in vitro. In contrast, treatment with lethal toxin (LT) from Clostridium sordellii, which predominantly inactivates Rac, and with toxin B from C. difficile, which inactivates Rho, Rac and Cdc42, decreased in vitro migration. When HPC pretreated with LT or toxin B were transplanted into mice, homing to the bone marrow was impaired, whereas C2I-C3 treatment did not alter HPC homing. However, in a competitive hematopoietic repopulation experiment in C57BL/6 mice, pretreatment of bone marrow cells with any of the inhibitors, including the Rho inhibitor C2I-C3, resulted in suppressed donor-type hematopoiesis. Our data indicate that whereas Rac supports HPC cell cycling, migration, short-term homing and hematopoietic regeneration, Rho coordinates down-regulation of HPC migration and is required for hematopoietic regeneration. |
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| DOI: | doi:10.1002/eji.200525607 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1002/eji.200525607 |
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| | DOI: https://doi.org/10.1002/eji.200525607 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | ADP Ribose Transferases |
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| | Animals |
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| | Botulinum Toxins |
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| | Cell Line |
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| | Cell Movement |
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| | Enzyme Activation |
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| | Enzyme Inhibitors |
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| | Hematopoietic Stem Cell Transplantation |
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| | Hematopoietic Stem Cells |
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| | Mice |
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| | rac GTP-Binding Proteins |
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| | rho GTP-Binding Proteins |
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| K10plus-PPN: | 1797137867 |
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| Verknüpfungen: | → Zeitschrift |
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Role of the monomeric GTPase Rho in hematopoietic progenitor cell migration and transplantation / Göttig, Stephan [VerfasserIn]; 2006 (Online-Ressource)
