GrinchGEF - a novel Rho-specific guanine nucleotide exchange factor

• Verfasst von: Winkler, Stefan [VerfasserIn]
• Verfasst von: Mohl, Marion [VerfasserIn]
• Verfasst von: Wieland, Thomas [VerfasserIn]
• Verfasst von: Lutz, Susanne [VerfasserIn]
• Titel: GrinchGEF - a novel Rho-specific guanine nucleotide exchange factor
• Verf.angabe: Stefan Winkler, Marion Mohl, Thomas Wieland, Susanne Lutz
• E-Jahr: 2005
• Jahr: 15 August 2005
• Umfang: 7 S.
• Fussnoten: Gesehen am 31.03.2022
• Titel Quelle: Enthalten in: Biochemical and biophysical research communications
• Ort Quelle: [Amsterdam] : Elsevier B.V., 1959
• Jahr Quelle: 2005
• Band/Heft Quelle: 335(2005), 4, Seite 1280-1286
• ISSN Quelle: 1090-2104
• DOI: doi:10.1016/j.bbrc.2005.08.025
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Amino Acid Sequence
• Sach-SW: Animals
• Sach-SW: Guanine Nucleotide Exchange Factors
• Sach-SW: Humans
• Sach-SW: Mice
• Sach-SW: Molecular Sequence Data
• Sach-SW: NIH 3T3 Cells
• Sach-SW: Organ Specificity
• Sach-SW: rho GTP-Binding Proteins
• Sach-SW: Rho Guanine Nucleotide Exchange Factors
• Sach-SW: Sequence Homology, Amino Acid
• Sach-SW: Tissue Distribution
• K10plus-PPN: 1797140647

Abstract

RhoGTPases, which are activated by specific guanine nucleotide exchange factors (GEFs), play pivotal roles in several cellular functions. We identified a new RhoGEF (GrinchGEF) containing the typical Dbl homology domain, a putative WD40-like domain, and two predicted transmembrane helices. In contrast to most other RhoGEFs, it exhibits no sequence similarities to known pleckstrin homology domains. GrinchGEF mRNA was highly abundant in skeletal muscle and pancreas. Despite the predicted transmembrane domains, subcellular localization studies revealed a cytosolic distribution. In vitro, GrinchGEF induced the GDP/GTP exchange at RhoA, but not at Rac1 or Cdc42. In intact cells, GrinchGEF induced specifically Rho activation and enhanced RhoA-C-specific downstream effects.