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| Verfasst von: | Fresnais, Margaux [VerfasserIn]  |
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| | Karabulut, Seda [VerfasserIn] |
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| | Abou Zeed, Yasmin [VerfasserIn] |
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| | Ungermann, Johannes [VerfasserIn] |
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| | Benzel, Julia [VerfasserIn] |
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| | Pajtler, Kristian Wilfried [VerfasserIn] |
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| | Pfister, Stefan [VerfasserIn] |
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| | Haefeli, Walter E. [VerfasserIn] |
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| | Burhenne, Jürgen [VerfasserIn] |
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| | Longuespée, Rémi [VerfasserIn] |
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| Titel: | Important requirements for the selection of internal standards during the development of desorption/ionization assays for drug quantification in biological matrices |
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| Titelzusatz: | a practical example |
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| Verf.angabe: | Margaux Fresnais, Seda Karabulut, Yasmin Abou Zeed, Johannes Ungermann, Julia Benzel, Kristian W. Pajtler, Stefan M. Pfister, Walter E. Haefeli, Jürgen Burhenne and Rémi Longuespée |
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| E-Jahr: | 2022 |
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| Jahr: | 21 January 2022 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 31.03.2022 |
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| Titel Quelle: | Enthalten in: Molecules |
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| Ort Quelle: | Basel : MDPI, 1996 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 27(2022), 3, Artikel-ID 690, Seite 1-13 |
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| ISSN Quelle: | 1420-3049 |
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| Abstract: | Desorption/ionization mass spectrometry (DI-MS) approaches allow for the rapid quantification of drugs in biological matrices using assays that can be validated according to regulatory guidelines. However, specific adaptations must be applied to create reliable quantification methods, depending on the approach and instrumentation used. In the present article, we demonstrate the importance of the molecular weight, the fragmentation pattern, and the purity of the internal standard for the development of matrix-assisted laser desorption/ionization (MALDI)-ion mobility (IM)-tandem MS and MS/MS methods. We present preliminary results of method development for the quantification of selinexor in microdialysis fluids with a stable isotopically labeled internal standard. In addition, we discuss the selection of internal standards for MALDI-MS assays using different instrumentations. |
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| DOI: | doi:10.3390/molecules27030690 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3390/molecules27030690 |
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| | Volltext: https://www.mdpi.com/1420-3049/27/3/690 |
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| | DOI: https://doi.org/10.3390/molecules27030690 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | biological matrices |
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| | desorption/ionization |
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| | drug |
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| | mass spectrometry |
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| | quantification |
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| K10plus-PPN: | 179714281X |
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| Verknüpfungen: | → Zeitschrift |
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Important requirements for the selection of internal standards during the development of desorption/ionization assays for drug quantification in biological matrices / Fresnais, Margaux [VerfasserIn]; 21 January 2022 (Online-Ressource)
