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| Verfasst von: | Steffens, Frank [VerfasserIn]  |
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| | Zhou, Xiao-Bo [VerfasserIn] |
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| | Sausbier, Ulrike [VerfasserIn] |
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| | Sailer, Claudia [VerfasserIn] |
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| | Motejlek, Karin [VerfasserIn] |
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| | Ruth, Peter [VerfasserIn] |
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| | Olcese, James [VerfasserIn] |
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| | Korth, Michael [VerfasserIn] |
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| | Wieland, Thomas [VerfasserIn] |
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| Titel: | Melatonin receptor signaling in pregnant and nonpregnant rat uterine myocytes as probed by large conductance Ca2+-activated K+ channel activity |
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| Verf.angabe: | Frank Steffens, Xiao-Bo Zhou, Ulrike Sausbier, Claudia Sailer, Karin Motejlek, Peter Ruth, James Olcese, Michael Korth, Thomas Wieland |
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| E-Jahr: | 2003 |
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| Jahr: | 01 October 2003 |
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| Umfang: | 13 S. |
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| Fussnoten: | Im Text ist "2" und jeweils "+" hochgestellt ; Gesehen am 07.04.2022 |
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| Titel Quelle: | Enthalten in: Molecular endocrinology |
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| Ort Quelle: | Bethesda, Md. : Endocrine Soc., 1987 |
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| Jahr Quelle: | 2003 |
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| Band/Heft Quelle: | 17(2003), 10, Seite 2103-2115 |
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| ISSN Quelle: | 1944-9917 |
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| Abstract: | The mRNAs of MT1 and MT2 melatonin receptors are present in cells from nonpregnant (NPM) and pregnant (PM) rat myometrium. To investigate the coupling of melatonin receptors to Gq- and Gi-type of heterotrimeric G proteins, we analyzed the activity of large-conductance Ca2+-activated K+ (BKCa) channels, the expression of which in the uterus is confined to smooth muscle cells. The melatonin receptor agonist 2-iodomelatonin induced a pertussis toxin (PTX)-insensitive increase in channel open probability that was blocked by the nonselective antagonist luzindole. The 2-iodomelatonin effect on channel open probability was suppressed by overexpression of the Gqalpha-inactivating protein RGS16 and the phospholipase C inhibitor U-73122. The activity of BKCa channels is differentially regulated by protein kinase A (PKA) in NPM and PM cells. Thus, the beta-adrenoceptor agonist isoprenaline inhibited the BKCa channel conducted whole-cell outward current (Iout) in NPM cells and enhanced Iout in PM cells. Additional application of 2-iodomelatonin antagonized the isoprenaline effect on Iout in NPM cells but enhanced Iout in PM cells. All 2-iodomelatonin effects on Iout were sensitive to PTX treatment and the PKA inhibitor H-89. We therefore conclude that melatonin activates both the PTX-insensitive Gq/phospholipase C/Ca2+ and the PTX-sensitive Gi/cAMP/PKA signaling pathway in rat myometrium. |
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| DOI: | doi:10.1210/me.2003-0047 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1210/me.2003-0047 |
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| | DOI: https://doi.org/10.1210/me.2003-0047 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Animals |
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| | Calcium |
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| | Cyclic AMP |
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| | Cyclic AMP-Dependent Protein Kinases |
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| | Female |
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| | GTP-Binding Protein alpha Subunits, Gi-Go |
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| | GTP-Binding Protein alpha Subunits, Gq-G11 |
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| | Isoproterenol |
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| | Large-Conductance Calcium-Activated Potassium Channels |
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| | Melatonin |
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| | Membrane Potentials |
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| | Myometrium |
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| | Pertussis Toxin |
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| | Potassium Channels, Calcium-Activated |
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| | Pregnancy |
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| | Rats |
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| | Rats, Wistar |
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| | Receptor, Melatonin, MT1 |
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| | Receptor, Melatonin, MT2 |
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| | Signal Transduction |
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| | Type C Phospholipases |
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| K10plus-PPN: | 1798095025 |
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| Verknüpfungen: | → Zeitschrift |
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Melatonin receptor signaling in pregnant and nonpregnant rat uterine myocytes as probed by large conductance Ca2+-activated K+ channel activity / Steffens, Frank [VerfasserIn]; 01 October 2003 (Online-Ressource)
