Breast cancer cell-derived cytokines, macrophages and cell adhesion

• Verfasst von: Eichbaum, Christine [VerfasserIn]
• Verfasst von: Meyer, Anne Sophie [VerfasserIn]
• Verfasst von: Wang, Ni [VerfasserIn]
• Verfasst von: Bischofs, Esther [VerfasserIn]
• Verfasst von: Steinborn-Kröhl, Andrea [VerfasserIn]
• Verfasst von: Bruckner, Thomas [VerfasserIn]
• Verfasst von: Brodt, Pnina [VerfasserIn]
• Verfasst von: Sohn, Christof [VerfasserIn]
• Verfasst von: Eichbaum, Michael H. R. [VerfasserIn]
• Titel: Breast cancer cell-derived cytokines, macrophages and cell adhesion
• Titelzusatz: implications for metastasis
• Verf.angabe: Christine Eichbaum, Anne-Sophie Meyer, Ni Wang, Esther Bischofs, Andrea Steinborn, Thomas Bruckner, Pnina Brodt, Christof Sohn and Michael H.R. Eichbaum
• E-Jahr: 2011
• Jahr: September 30, 2011
• Umfang: 9 S.
• Fussnoten: Gesehen am 08.04.2022
• Titel Quelle: Enthalten in: Anticancer research
• Ort Quelle: Attiki : [Verlag nicht ermittelbar], 2004
• Jahr Quelle: 2011
• Band/Heft Quelle: 31(2011), 10, Seite 3219-3227
• ISSN Quelle: 1791-7530
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Breast cancer
• Sach-SW: E-selectin
• Sach-SW: endothelium
• Sach-SW: liver metastasis
• Sach-SW: pro-inflammatory microenvironment
• K10plus-PPN: 1798277867

Abstract

Background: Liver metastasis is associated with a proinflammatory microenvironment and up-regulation of cell adhesion molecules expressed by endothelial cells. The aim of this study was to characterize the interrelations between breast cancer cell-secreted cytokines, macrophages and E-selectin-mediated cancer cell adhesion and their role in metastasis of breast cancer. Materials and Methods: Three metastatic breast cancer cell lines (1590, KM22, ZE) were studied. Cell culture supernatants were screened for cytokines and the potential for cytokines to increase tumor-necrosis factor-α (TNF-α) production by ANA-1-macrophages was analyzed. E-Selectin-mediated tumor cell adhesion of fluorescence labelled tumor cells was evaluated by measurement of fluorescence intensity with and without E-selectin-blocking strategies (monoclonal antibodies, cimetidine). Results: Tumor-specific cytokine secretion patterns were revealed. TNF-α secretion from cultured macrophages increased after incubation with tumor supernatants. Tumor cell adhesion was significantly inhibited by cimetidine and monoclonal antibodies against E-selectin (KM22 with cimetidine, p<0.05). Conclusion: Breast cancer cell-secreted cytokines stimulate macrophages to produce TNF-α, a known up-regulator of E-selectin expression, and therefore cell adherence to endothelium. Inhibition of this mechanism could be an attractive therapeutic option for the prevention of breast cnacer metastasis.