Specific progesterone binding to a membrane protein and related nongenomic effects on Ca2+-fluxes in sperm

• Verfasst von: Falkenstein, Elisabeth [VerfasserIn]
• Verfasst von: Heck, Marina [VerfasserIn]
• Verfasst von: Gerdes, Dirk [VerfasserIn]
• Verfasst von: Grube, Daniel [VerfasserIn]
• Verfasst von: Christ, Michael [VerfasserIn]
• Verfasst von: Weigel, Michael [VerfasserIn]
• Verfasst von: Buddhikot, Madhavee [VerfasserIn]
• Verfasst von: Meizel, Stanley [VerfasserIn]
• Verfasst von: Wehling, Martin [VerfasserIn]
• Titel: Specific progesterone binding to a membrane protein and related nongenomic effects on Ca2+-fluxes in sperm
• Verf.angabe: Elisabeth Falkenstein, Marina Heck, Dirk Gerdes, Daniel Grube, Michael Christ, Michael Weigel, Madhavee Buddhikot, Stanley Meizel and Martin Wehling
• E-Jahr: 1999
• Jahr: 01 December 1999
• Umfang: 4 S.
• Fussnoten: Im Text ist "2+" hochgestellt ; Gesehen am 11.04.2022
• Titel Quelle: Enthalten in: Endocrinology
• Ort Quelle: Oxford : Oxford University Press, 1917
• Jahr Quelle: 1999
• Band/Heft Quelle: 140(1999), 12, Seite 5999-6002
• ISSN Quelle: 1945-7170
• DOI: doi:10.1210/endo.140.12.7304
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1798899523

Abstract

Rapid, nongenomic effects of steroids are supposed to be transmitted by membrane receptors unrelated to the classic intracellular steroid receptors. In this context, a putative progesterone membrane binding protein (mPR) has been identified, recently. Here we show that expression of mPR-cDNA in CHO cells leads to increased microsomal progesterone binding. This result is mirrored by effects of an antibody raised against the recombinant E. coli mPR which suppressed the rapid progesterone-initiated Ca2+ increase in sperm. Our results support the assumption that mPR represents the first steroid membrane receptor or a part of it involved in rapid, nongenomic steroid signalling.