| |  Online-Ressource |
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| Verfasst von: | Walch, Philipp [VerfasserIn]  |
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| | Selkrig, Joel [VerfasserIn] |
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| | Knodler, Leigh A. [VerfasserIn] |
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| | Rettel, Mandy [VerfasserIn] |
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| | Stein, Frank [VerfasserIn] |
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| | Fernandez, Keith [VerfasserIn] |
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| | Viéitez, Cristina [VerfasserIn] |
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| | Potel, Clément M. [VerfasserIn] |
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| | Scholzen, Karoline [VerfasserIn] |
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| | Geyer, Matthias [VerfasserIn] |
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| | Rottner, Klemens [VerfasserIn] |
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| | Steele-Mortimer, Olivia [VerfasserIn] |
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| | Savitski, Mikhail M. [VerfasserIn] |
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| | Holden, David W. [VerfasserIn] |
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| | Typas, Athanasios [VerfasserIn] |
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| Titel: | Global mapping of Salmonella enterica-host protein-protein interactions during infection |
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| Verf.angabe: | Philipp Walch, Joel Selkrig, Leigh A. Knodler, Mandy Rettel, Frank Stein, Keith Fernandez, Cristina Viéitez, Clément M. Potel, Karoline Scholzen, Matthias Geyer, Klemens Rottner, Olivia Steele-Mortimer, Mikhail M. Savitski, David W. Holden, and Athanasios Typas |
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| E-Jahr: | 2021 |
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| Jahr: | 7 July 2021 |
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| Umfang: | 17 S. |
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| Fussnoten: | Gesehen am 14.04.2022 |
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| Titel Quelle: | Enthalten in: Cell host and microbe |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier, 2007 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 29(2021), 8, Seite 1316-1332.e12 |
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| ISSN Quelle: | 1934-6069 |
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| Abstract: | Intracellular bacterial pathogens inject effector proteins to hijack host cellular processes and promote their survival and proliferation. To systematically map effector-host protein-protein interactions (PPIs) during infection, we generated a library of 32 Salmonella enterica serovar Typhimurium (STm) strains expressing chromosomally encoded affinity-tagged effectors and quantified PPIs in macrophages and epithelial cells. We identified 446 effector-host PPIs, 25 of which were previously described, and validated 13 by reciprocal co-immunoprecipitation. While effectors converged on the same host cellular processes, most had multiple targets, which often differed between cell types. We demonstrate that SseJ, SseL, and SifA modulate cholesterol accumulation at the Salmonella-containing vacuole (SCV) partially via the cholesterol transporter Niemann-Pick C1 protein. PipB recruits the organelle contact site protein PDZD8 to the SCV, and SteC promotes actin bundling by phosphorylating formin-like proteins. This study provides a method for probing host-pathogen PPIs during infection and a resource for interrogating STm effector mechanisms. |
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| DOI: | doi:10.1016/j.chom.2021.06.004 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.chom.2021.06.004 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S1931312821002821 |
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| | DOI: https://doi.org/10.1016/j.chom.2021.06.004 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | actin bundling |
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| | bacterial pathogen |
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| | cholesterol trafficking |
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| | effectors |
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| | FMNL |
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| | NPC1 |
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| | PDZD8 |
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| | protein-protein interactions |
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| K10plus-PPN: | 1799642070 |
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| Verknüpfungen: | → Zeitschrift |
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Global mapping of Salmonella enterica-host protein-protein interactions during infection / Walch, Philipp [VerfasserIn]; 7 July 2021 (Online-Ressource)
