| |  Online-Ressource |
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| Verfasst von: | Dalakouras, Athanasios [VerfasserIn]  |
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| | Moser, Mirko [VerfasserIn] |
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| | Boonrod, Kajohn [VerfasserIn] |
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| | Krczal, Gabi [VerfasserIn] |
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| | Wassenegger, Michael [VerfasserIn] |
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| Titel: | Diverse spontaneous silencing of a transgene among two Nicotiana species |
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| Verf.angabe: | Athanasios Dalakouras, Mirko Moser, Kajohn Boonrod, Gabi Krczal, Michael Wassenegger |
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| E-Jahr: | 2011 |
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| Jahr: | 27 May 2011 |
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| Umfang: | 9 S. |
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| Fussnoten: | Gesehen am 25.04.2022 |
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| Titel Quelle: | Enthalten in: Planta |
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| Ort Quelle: | Berlin : Springer, 1925 |
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| Jahr Quelle: | 2011 |
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| Band/Heft Quelle: | 234(2011), 4, Seite 699-707 |
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| ISSN Quelle: | 1432-2048 |
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| Abstract: | In plants, transgenes frequently become spontaneously silenced for unknown reasons. Typically, transgene silencing involves the generation of small interfering RNAs (siRNAs) that directly or indirectly target cognate DNA and mRNA sequences for methylation and degradation, respectively. In this report, we compared spontaneous silencing of a transgene in Nicotiana benthamiana and Nicotiana tabacum. In both species, abundant siRNAs were produced. In N. benthamiana, the self-silencing process involved mRNA degradation and dense DNA methylation of the homologous coding region. In N.tabacum, self-silencing occurred without complete mRNA degradation and with low methylation of the cognate coding region. Our data indicated that in plants, siRNA-mediated spontaneous silencing is, in addition to mRNA degradation, based on translational inhibition. Differences in the initiation and establishment of self-silencing together with marked differences in the degree of de novo DNA methylation showed that the mechanistic details of RNA silencing, although largely conserved, may vary also in genetically close plant species. |
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| DOI: | doi:10.1007/s00425-011-1433-9 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1007/s00425-011-1433-9 |
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| | Volltext: https://link.springer.com/article/10.1007/s00425-011-1433-9 |
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| | DOI: https://doi.org/10.1007/s00425-011-1433-9 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Nicotiana benthamiana |
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| | Nicotiana tabacum |
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| | RNA-directed DNA methylation |
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| | Small interfering RNA |
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| | Spontaneous silencing |
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| K10plus-PPN: | 1800219083 |
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| Verknüpfungen: | → Zeitschrift |
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Diverse spontaneous silencing of a transgene among two Nicotiana species / Dalakouras, Athanasios [VerfasserIn]; 27 May 2011 (Online-Ressource)
