Improved laboratory confirmation of heparin-induced thrombocytopenia type II

• Verfasst von: Harenberg, Job [VerfasserIn]
• Verfasst von: Wang, Lianchun [VerfasserIn]
• Verfasst von: Hoffmann, Ursula [VerfasserIn]
• Verfasst von: Huhle, Günter [VerfasserIn]
• Verfasst von: Feuring, Martin [VerfasserIn]
• Titel: Improved laboratory confirmation of heparin-induced thrombocytopenia type II
• Titelzusatz: time course of antibodies and combination of antigen and biologic assays
• Verf.angabe: Job Harenberg, Lianchun Wang, Ursula Hoffmann, Günter Huhle, Martin Feuring
• E-Jahr: 2001
• Jahr: 03 January 2001
• Umfang: 7 S.
• Fussnoten: Gesehen am 26.04.2022
• Titel Quelle: Enthalten in: American journal of clinical pathology
• Ort Quelle: Chicago, Ill. : Soc., 1931
• Jahr Quelle: 2001
• Band/Heft Quelle: 115(2001), 3, Seite 432-438
• ISSN Quelle: 1943-7722
• DOI: doi:10.1309/ET2W-5J8E-MFVU-QYAH
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 180029459X

Abstract

We studied whether laboratory confirmation of heparin-induced thrombocytopenia (HIT) can be improved after antigen clearance by determining free antibody and combining the results of an antigenic and a biologic assay. Blood samples taken over 40 days in 14 patients with HIT with thromboembolism underwent fluorescence-linked immunofiltration and the carbon 14-serotonin release assays.Of the 14 patients, 11 showed positive results in both assays at day 1 after stopping heparin. The 3 patients with negative results seroconverted in one or both assays during the subsequent 7 days. Combining the positive results of the assays increased the sensitivity from 85% at day 1 to 100% at day 7. Assay results became negative in all patients within 40 days. The platelet count normalized between days 2 and 9 after withdrawal of heparin. It is assumed that the free antibody can be detected after withdrawal of heparin and after clearance of the platelet factor 4-heparin complex in patients with HIT.