Functional activity of HERV-K-T47D-related long terminal repeats

• Verfasst von: Baust, Corinna [VerfasserIn]
• Verfasst von: Seifarth, Wolfgang [VerfasserIn]
• Verfasst von: Schön, Ulrike [VerfasserIn]
• Verfasst von: Hehlmann, Rüdiger [VerfasserIn]
• Verfasst von: Leib-Mösch, Christine [VerfasserIn]
• Titel: Functional activity of HERV-K-T47D-related long terminal repeats
• Verf.angabe: Corinna Baust, Wolfgang Seifarth, Ulrike Schön, Rüdiger Hehlmann, and Christine Leib-Mösch
• Jahr: 2001
• Umfang: 11 S.
• Fussnoten: Elektronische Reproduktion der Druckausgabe ; Gesehen am 18.05.2022
• Titel Quelle: Enthalten in: Virology
• Ort Quelle: San Diego, Calif. [u.a.] : Elsevier, 1955
• Jahr Quelle: 2001
• Band/Heft Quelle: 283(2001), 2, Seite 262-272
• ISSN Quelle: 1096-0341
• DOI: doi:10.1006/viro.2001.0898
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: HERV-K-T47D
• Sach-SW: human endogenous retrovirus
• Sach-SW: LTR
• Sach-SW: transcriptional regulation
• K10plus-PPN: 1802480196

Abstract

The human genome contains a family of endogenous retroviruses, HERV-K(HML-4), that comprises the full-length provirus HERV-K-T47D, five related elements, and hundreds of solitary long terminal repeats (LTRs). We here show that HERV-K-T47D-related LTRs are dispersed over all human chromosomes and have arisen after the divergence of Old and New World monkeys. By screening a cDNA library derived from the human mammary carcinoma cell line T47D with a HERV-K-T47D LTR probe, we isolated several clones containing LTR/cellular gene chimeras and assessed the transcriptional activity of these LTRs in transient transfection experiments. All LTRs were able to drive the expression of a reporter gene, thereby displaying distinct activities in different cell lines. We found that sequences located downstream of the LTR-U3 region modulate the level of gene expression. Based on the impact of the R region we distinguished between three different LTR types; the activity of type I LTRs was enhanced in the presence of the LTR-R region in all cell lines tested, whereas a type II LTR was downregulated. Type III LTRs are characterized by lacking or having a varying influence of the R region that was dependent on the cell line used. Finally, our results attribute to LTR-U5-gag sequences a role in determining LTR activity.