Monitoring of residual disease in patients with chronic myelogenous leukemia using specific fluorescent hybridization probes for real-time quantitative RT-PCR

• Verfasst von: Hochhaus, Andreas [VerfasserIn]
• Verfasst von: Emig, Michael [VerfasserIn]
• Verfasst von: Weißer, Andreas [VerfasserIn]
• Verfasst von: Saußele, Susanne [VerfasserIn]
• Verfasst von: Müller, Martin Christian [VerfasserIn]
• Verfasst von: La Rosée, Paul [VerfasserIn]
• Verfasst von: Kuhn, Christian [VerfasserIn]
• Verfasst von: Paschka, Peter [VerfasserIn]
• Verfasst von: Hehlmann, Rüdiger [VerfasserIn]
• Titel: Monitoring of residual disease in patients with chronic myelogenous leukemia using specific fluorescent hybridization probes for real-time quantitative RT-PCR
• Verf.angabe: Andreas Hochhaus, Michael Emig, Andreas Weisser, Susanne Saussele, Martin Müller, Paul La Rosée, Christian Kuhn, Peter Paschka & Rüdiger Hehlmann
• Jahr: 2001
• Umfang: 15 S.
• Fussnoten: Gesehen am 18.05.2022
• Titel Quelle: Enthalten in: Meuer, Stefan, 1951 - Rapid Cycle Real-Time PCR
• Ort Quelle: Berlin, Heidelberg : Springer Berlin Heidelberg, 2001
• Jahr Quelle: 2001
• Band/Heft Quelle: (2001), Seite 171-185
• ISBN Quelle: 978-3-642-59524-0
• DOI: doi:10.1007/978-3-642-59524-0_20
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1802494456

Abstract

In many ways, chronic myelogenous leukemia (CML) serves as a paradigm for the utility of molecular methods to diagnose malignancy or to monitor patient response to therapy [1]. CML constitutes a clinical model for molecular detection and therapy surveillance since this entity was the first leukemia known to be associated with a specific chromosomal rearrangement, the Philadelphia (Ph) translocation t(9;22)(q34;q11), and the presence of two chimeric genes, BCR-ABL on chromosome 22 and ABL-BCR on chromosome 9. BCR-ABL is transcribed to a specific BCR-ABL mRNA and encodes in most patients a 210-kDa chimeric protein with increased tyrosine kinase activity. The central role of BCR-ABL in several pathways which lead to uncontrolled proliferation has been shown in vitro and in vivo. Several approaches have been introduced that can specifically detect the Ph translocation or its products, such as fluorescent in situ hybridization, Southern blotting, western blotting, and reverse transcriptase polymerase chain reaction (RT-PCR) [2–4]. Of these, RT-PCR for BCR-ABL mRNA is by far the most sensitive and consequently has received the most attention in the context of minimal residual disease.