CD14+ peripheral blood mononuclear cells from chronic myeloid leukemia and normal donors are inhibitory to short- and long-term cultured colony-forming cells

• Verfasst von: Heissig, Beate [VerfasserIn]
• Verfasst von: Pasternak, Günter [VerfasserIn]
• Verfasst von: Hörner, Susanne [VerfasserIn]
• Verfasst von: Schwerdtfeger, Rainer [VerfasserIn]
• Verfasst von: Rossol, Siegfried [VerfasserIn]
• Verfasst von: Hehlmann, Rüdiger [VerfasserIn]
• Titel: CD14+ peripheral blood mononuclear cells from chronic myeloid leukemia and normal donors are inhibitory to short- and long-term cultured colony-forming cells
• Verf.angabe: Beate Heissig, Günther Pasternak, Susanne Hörner, Rainer Schwerdtfeger, Siegfried Rossol, Rüdiger Hehlmann
• E-Jahr: 2000
• Jahr: 11 February 2000
• Umfang: 15 S.
• Fussnoten: Gesehen am 19.05.2022 ; Im Titel ist das Pluszeichen hochgestellt
• Titel Quelle: Enthalten in: Leukemia research
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1977
• Jahr Quelle: 2000
• Band/Heft Quelle: 24(2000), 3, Seite 217-231
• ISSN Quelle: 1873-5835
• DOI: doi:10.1016/S0145-2126(99)00171-X
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: CML
• Sach-SW: Colony-forming cells
• Sach-SW: Haematopoiesis
• Sach-SW: Long-term culture
• Sach-SW: Monocytes
• Sach-SW: Short-term culture
• K10plus-PPN: 1802597158

Abstract

Monocyte-induced cell-cytotoxicity has been implicated in the mechanism of suppression of normal haematopoietic progenitors in chronic myeloid leukemia (CML). We examined here the in vitro effect of CML-derived and normal peripheral blood (PB) monocytes on short- and long-term cultured haematopoietic progenitor cells. Short-term coculture (5 days) of CML or normal monocytes with CML or normal peripheral blood mononuclear cells (PBMNC)/CD34+ cells as targets resulted in a significant inhibition of colony-forming cell (CFC) growth. Coculture conditioned medium (CCM) from 5-days cocultures of normal or CML CD14+ monocytes with CD34+ cells were likewise inhibitory to CFC. In 5-week long-term cocultures of monocytes in direct contact with normal bone marrow (BM) progenitors, CML monocytes reduced the proportion of long-term cultured CFC (LTC-CFC) significantly to 52% of the controls, while normal monocytes had a less pronounced inhibitory effect (89% of the controls) on LTC-CFC. Reduction of LTC-CFC was great when CML monocytes and target cells were separated by a transwell membrane as compared to control cultures in the absence of CD14+ cells (53.5 vs. 9%). CCM from 5-week cocultures of normal or CML CD14+ monocytes with CD34+ progenitors from bone marrow (BM) cells were also inhibitory to CFC. No difference in cytokine levels for TNF-α, IFN-γ, G-CSF, IL-10, IL-6 was detectable between CML CD14+ CCM and control CCM derived from short- and long-term cocultures. Our results suggest that CML monocytes may play a role in the inhibition of normal haematopoiesis through a yet not defined soluble factor supporting the expansion of the malignant clone in CML.