Does long-term culture favor normal clonogenic cells from interferon-treated patients with chronic myelogenous leukemia?

• Verfasst von: Pasternak, Günter [VerfasserIn]
• Verfasst von: Schultheis, Beate [VerfasserIn]
• Verfasst von: Heissig, Beate [VerfasserIn]
• Verfasst von: Hörner, Susanne [VerfasserIn]
• Verfasst von: Sick, Christian [VerfasserIn]
• Verfasst von: Hehlmann, Rüdiger [VerfasserIn]
• Titel: Does long-term culture favor normal clonogenic cells from interferon-treated patients with chronic myelogenous leukemia?
• Verf.angabe: G. Pasternak, B. Schultheis, B. Heissig, S. Hörner, C. Sick and R. Hehlmann
• E-Jahr: 1999
• Jahr: 15 June 1999
• Umfang: 10 S.
• Fussnoten: Gesehen am 19.05.2022
• Titel Quelle: Enthalten in: Leukemia
• Ort Quelle: London : Springer Nature, 1997
• Jahr Quelle: 1999
• Band/Heft Quelle: 13(1999), 1, Seite S55-S64
• ISSN Quelle: 1476-5551
• DOI: doi:10.1038/sj.leu.2401288
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Cancer Research
• Sach-SW: general
• Sach-SW: Hematology
• Sach-SW: Intensive / Critical Care Medicine
• Sach-SW: Internal Medicine
• Sach-SW: Medicine/Public Health
• Sach-SW: Oncology
• K10plus-PPN: 1803355506

Abstract

We have tested whether peripheral blood mononuclear cells (PBMNCs) from interferon (IFN)-treated patients may lose residual BCR-ABL sequence-positive progenitor cells when long-term cultured for 35 days on allogeneic stromal cells. IFN-treated patients have low white blood cell counts and a fair number of BCR-ABL-negative colony-forming cells in the peripheral blood. Particularly, IFN responders show increased numbers of normal hematopoietic cells. We have quantitatively analyzed progenitor cells in PBMNCs of IFN-treated patients by combining the clonogenic assay in semisolid medium with interphase fluorescent in situ hybridization (FISH). Thus, the identification is possible of the BCR-ABL status of colony-forming progenitor cells. In IFN-treated patients, the number of BCR-ABL-positive CFCs is considerably decreased and BCR-ABL-negative CFCs appear in the peripheral blood. We could show that after LTC for 35 days of the same PBMNCs on irradiated allogeneic normal stromal cells residual BCR-ABL sequence-positive CFCs were still present. In some cases the relative number of BCR-ABL sequence-positive CFCs was found to be increased after LTC. A minor proportion of blood samples from IFN-treated patients did not give rise to CFCs after LTC on allogeneic stromal cells (three of 10 patients). Inter- and intraindividual variations can be found with regard to loss or gain of BCR-ABL sequence-positive colonies after LTC. We conclude that early CML progenitor cells persist in the peripheral blood of IFN-treated patients and that a certain proportion may survive long-term culture.