Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Weißenbruch, Kai [VerfasserIn]  |
| Fladung, Magdalena [VerfasserIn]  |
| Grewe, Justin [VerfasserIn]  |
| Baulesch, Laurent [VerfasserIn]  |
| Schwarz, Ulrich S. [VerfasserIn]  |
| Bastmeyer, Martin [VerfasserIn]  |
Titel: | Nonmuscle myosin IIA dynamically guides regulatory light chain phosphorylation and assembly of nonmuscle myosin IIB |
Verf.angabe: | Kai Weißenbruch, Magdalena Fladung, Justin Grewe, Laurent Baulesch, Ulrich S. Schwarz, Martin Bastmeyer |
E-Jahr: | 2022 |
Jahr: | 2 March 2022 |
Umfang: | 15 S. |
Fussnoten: | Gesehen am 26.10.2022 |
Titel Quelle: | Enthalten in: European journal of cell biology |
Ort Quelle: | München : Elsevier, 1998 |
Jahr Quelle: | 2022 |
Band/Heft Quelle: | 101(2022), 2, Artikel-ID 151213, Seite 1-15 |
ISSN Quelle: | 1618-1298 |
Abstract: | Nonmuscle myosin II minifilaments have emerged as central elements for force generation and mechanosensing by mammalian cells. Each minifilament can have a different composition and activity due to the existence of the three nonmuscle myosin II paralogs A, B and C and their respective phosphorylation pattern. We have used CRISPR/Cas9-based knockout cells, quantitative image analysis and mathematical modeling to dissect the dynamic processes that control the formation and activity of heterotypic minifilaments and found a strong asymmetry between paralogs A and B. Loss of NM IIA completely abrogates regulatory light chain phosphorylation and reduces the level of assembled NM IIB. Activated NM IIB preferentially co-localizes with pre-formed NM IIA minifilaments and stabilizes the filament in a force-dependent mechanism. NM IIC is only weakly coupled to these processes. We conclude that NM IIA and B play clearly defined complementary roles during assembly of functional minifilaments. NM IIA is responsible for the formation of nascent pioneer minifilaments. NM IIB incorporates into these and acts as a clutch that limits the force output to prevent excessive NM IIA activity. Together these two paralogs form a balanced system for regulated force generation. |
DOI: | doi:10.1016/j.ejcb.2022.151213 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.ejcb.2022.151213 |
| Volltext: https://www.sciencedirect.com/science/article/pii/S0171933522000164 |
| DOI: https://doi.org/10.1016/j.ejcb.2022.151213 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Actomyosin |
| Cellular force generation |
| Mechanobiology |
| Minifilaments |
| Nonmuscle myosin II |
| Paralogs |
K10plus-PPN: | 180409370X |
Verknüpfungen: | → Zeitschrift |
Nonmuscle myosin IIA dynamically guides regulatory light chain phosphorylation and assembly of nonmuscle myosin IIB / Weißenbruch, Kai [VerfasserIn]; 2 March 2022 (Online-Ressource)
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