| |  Online-Ressource |
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| Verfasst von: | Wang, Lianchun [VerfasserIn]  |
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| | Huhle, Günter [VerfasserIn] |
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| | Malsch, Reinhard [VerfasserIn] |
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| | Hoffmann, Ursula [VerfasserIn] |
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| | Song, Xuehong [VerfasserIn] |
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| | Harenberg, Job [VerfasserIn] |
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| Titel: | Determination of heparin-induced IgG antibody by fluorescence-linked immunofiltration assay (FLIFA) |
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| Verf.angabe: | Lianchun Wang, Guenter Huhle, Reinhard Malsch, Ursula Hoffmann, Xuehong Song, Job Harenberg |
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| Jahr: | 1999 |
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| Umfang: | 7 S. |
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| Fussnoten: | Available online 22 December 1998 ; Gesehen am 24.05.2022 |
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| Titel Quelle: | Enthalten in: Journal of immunological methods |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1971 |
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| Jahr Quelle: | 1999 |
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| Band/Heft Quelle: | 222(1999), 1, Seite 93-99 |
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| ISSN Quelle: | 1872-7905 |
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| Abstract: | A fluorescence-linked immunofiltration assay (FLIFA) was developed for the determination of heparin-induced IgG in heparin-induced thrombocytopenia (HIT) type II patients. Protein A was immobilized on a nitrocellulose membrane to bind heparin-induced IgG of HIT type II patients. Fluorescein-5-isothiocynate (FITC)-heparin was added to platelet factor 4 present in normal serum to form the neo-antigen which was captured by heparin-induced IgG. The heparin-induced IgG was quantified by the relative fluorescence intensity (RFI) of bound FITC-heparin. Values were expressed as a RFI ratio (RFI patient / RFI normal) and were 1.965±0.413 in HIT type II patients (n=36) and 1.064±0.162 in healthy controls (n=50, p<0.0001). The intra- and inter-assay coefficients of variation were 4.9 and 10.4%, respectively. The heparin-induced IgG FLIFA will be useful in individual and epidemiological studies in patients during treatment with heparin. The FLIFA technique offers an alternative, rapid and sensitive methodological approach for studies on the interaction between antigen-antibody or ligand-receptor. |
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| DOI: | doi:10.1016/S0022-1759(98)00186-0 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/S0022-1759(98)00186-0 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S0022175998001860 |
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| | DOI: https://doi.org/10.1016/S0022-1759(98)00186-0 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Antibody |
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| | Fluorescence linked immunofiltration assay |
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| | Heparin-induced thrombocytopenia |
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| | Relative fluorescence intensity |
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| K10plus-PPN: | 1804249459 |
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| Verknüpfungen: | → Zeitschrift |
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Determination of heparin-induced IgG antibody by fluorescence-linked immunofiltration assay (FLIFA) / Wang, Lianchun [VerfasserIn]; 1999 (Online-Ressource)
