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Verfasst von:Löhr, J.-Matthias [VerfasserIn]   i
 Müller, Petra [VerfasserIn]   i
 Zauner, Ira [VerfasserIn]   i
 Schmidt, Christian [VerfasserIn]   i
 Trautmann, Birgit [VerfasserIn]   i
 Thévenod, Frank [VerfasserIn]   i
 Capellα, Gabriel [VerfasserIn]   i
 Farré, Antonio [VerfasserIn]   i
 Liebe, Stefan [VerfasserIn]   i
 Jesenofsky, Ralf [VerfasserIn]   i
Titel:Immortalized bovine pancreatic duct cells become tumorigenic after transfection with mutant k-ras
Verf.angabe:Matthias Löhr, Petra Müller, Ira Zauner, Christian Schmidt, Birgit Trautmann, Frank Thévenod, Gabriel Capellα, Antonio Farré, Stefan Liebe, Ralf Jesnowski
E-Jahr:2001
Jahr:02 March 2001
Umfang:10 S.
Fussnoten:Gesehen am 24.05.2022
Titel Quelle:Enthalten in: Virchows Archiv
Ort Quelle:Berlin : Springer, 1847
Jahr Quelle:2001
Band/Heft Quelle:438(2001), 6, Seite 581-590
ISSN Quelle:1432-2307
Abstract:Mutation of the K-ras gene is thought to be an early and important event in pancreatic carcinogenesis. In order to study the role of this molecular alteration in the transition from the normal to the neoplastic pancreatic cell, bovine pancreatic duct cells were first immortalized by SV40 large T antigen (Ag) complementary (c)DNA transfection and then transfected with a mutated K-ras gene. As did primary duct cells, the immortalized duct cells (more than 100 passages) expressed cytokeratins, carbonic anhydrase type-II, cystic fibrosis transmembrane conductance regulator (CFTR), and multidrug resistance (mdr). They grew as a single layer after transplantation under plastic domes and formed three-dimensional structures resembling ducts when grown on Matrigel. Cell growth was stimulated by insulin, epidermal growth factor (EGF), transforming growth factor (TGF)-α, but cells did not respond to gastrin and CCK-8. They did not form colonies in soft agar nor did they form tumors in nude mice. Immortalized cells transfected with mutated K-ras acquired the ability to form tumors after orthotopic injection into the nude mouse pancreas. It is concluded that SV 40 immortalized bovine pancreatic duct cells retain the features of normal duct cells and gain tumorigenicity by transfection with mutated K-ras. This suggests an important role for K-ras in this pancreatic carcinoma model.
DOI:doi:10.1007/s004280100397
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1007/s004280100397
 DOI: https://doi.org/10.1007/s004280100397
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Carcinogenesis
 Immortalization
 K-ras transfection
 Pancreatic duct cell
 SV40
 Tumorigenicity
K10plus-PPN:1804291218
Verknüpfungen:→ Zeitschrift

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