Ribosomal highly basic 23-kDa protein as a reliable standard for gene expression analysis

• Verfasst von: Jesenofsky, Ralf [VerfasserIn]
• Verfasst von: Backhaus, C. [VerfasserIn]
• Verfasst von: Ringel, Jörg [VerfasserIn]
• Verfasst von: Löhr, J.-Matthias [VerfasserIn]
• Titel: Ribosomal highly basic 23-kDa protein as a reliable standard for gene expression analysis
• Verf.angabe: R. Jesnowski, C. Backhaus, J. Ringel, M. Löhr
• Jahr: 2002
• Umfang: 4 S.
• Fussnoten: Elektronische Reproduktion der Druckausgabe ; Gesehen am 24.05.2022
• Titel Quelle: Enthalten in: Pancreatology
• Ort Quelle: Amsterdam : Elsevier, 2001
• Jahr Quelle: 2002
• Band/Heft Quelle: 2(2002), 4, Seite 421-424
• ISSN Quelle: 1424-3911
• DOI: doi:10.1159/000065091
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Housekeeping
• Sach-SW: Internal standard
• Sach-SW: RNA W Gene expression
• K10plus-PPN: 1804291773

Abstract

Background/Aims: Analysis of gene expression is dependent on normalization using housekeeping genes. However, many of these housekeeping genes (e.g. GAPDH, ß-actin) are upregulated in chronic pancreatitis and pancreatic cancer, and cannot be used for normalization. For this reason we tried to identify a housekeeping gene useful for expression analysis in pancreatic diseases. Methods: RNA isolated from various tissues and states of disease was subjected to reverse transcription and subsequently amplified by PCR using primers for GAPDH and for the ribosomal highly basic 23-kDa (rb 23kDa, RPL13A) protein. Results: As anticipated, expression of GAPDH varied markedly in the different tissues, whereas the expression of rb 23-kDa was constant in all samples investigated. Conclusion: We recommend the use of the ribosomal highly basic 23-kDa protein as a standard for normalization at least for the pancreas and the prostate.