Status: Bibliographieeintrag
Standort: ---
Exemplare:
---
| Online-Ressource |
Verfasst von: | Jesenofsky, Ralf [VerfasserIn]  |
| Backhaus, C. [VerfasserIn]  |
| Ringel, Jörg [VerfasserIn]  |
| Löhr, J.-Matthias [VerfasserIn]  |
Titel: | Ribosomal highly basic 23-kDa protein as a reliable standard for gene expression analysis |
Verf.angabe: | R. Jesnowski, C. Backhaus, J. Ringel, M. Löhr |
Jahr: | 2002 |
Umfang: | 4 S. |
Fussnoten: | Elektronische Reproduktion der Druckausgabe ; Gesehen am 24.05.2022 |
Titel Quelle: | Enthalten in: Pancreatology |
Ort Quelle: | Amsterdam : Elsevier, 2001 |
Jahr Quelle: | 2002 |
Band/Heft Quelle: | 2(2002), 4, Seite 421-424 |
ISSN Quelle: | 1424-3911 |
Abstract: | Background/Aims: Analysis of gene expression is dependent on normalization using housekeeping genes. However, many of these housekeeping genes (e.g. GAPDH, ß-actin) are upregulated in chronic pancreatitis and pancreatic cancer, and cannot be used for normalization. For this reason we tried to identify a housekeeping gene useful for expression analysis in pancreatic diseases. Methods: RNA isolated from various tissues and states of disease was subjected to reverse transcription and subsequently amplified by PCR using primers for GAPDH and for the ribosomal highly basic 23-kDa (rb 23kDa, RPL13A) protein. Results: As anticipated, expression of GAPDH varied markedly in the different tissues, whereas the expression of rb 23-kDa was constant in all samples investigated. Conclusion: We recommend the use of the ribosomal highly basic 23-kDa protein as a standard for normalization at least for the pancreas and the prostate. |
DOI: | doi:10.1159/000065091 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1159/000065091 |
| Volltext: https://www.sciencedirect.com/science/article/pii/S1424390302800412 |
| DOI: https://doi.org/10.1159/000065091 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Housekeeping |
| Internal standard |
| RNA W Gene expression |
K10plus-PPN: | 1804291773 |
Verknüpfungen: | → Zeitschrift |
Ribosomal highly basic 23-kDa protein as a reliable standard for gene expression analysis / Jesenofsky, Ralf [VerfasserIn]; 2002 (Online-Ressource)
68922091