Altered CD46-mediated T cell co-stimulation in haemodialysis patients

• Verfasst von: Brinkkötter, Paul-Thomas [VerfasserIn]
• Verfasst von: Marinaki, Smaragdi [VerfasserIn]
• Verfasst von: Göttmann, Uwe [VerfasserIn]
• Verfasst von: Fleckenstein, Sandra Magdalena [VerfasserIn]
• Verfasst von: Stump-Guthier, Carolin [VerfasserIn]
• Verfasst von: Woude, Fokko J. van der [VerfasserIn]
• Verfasst von: Braun, Claude [VerfasserIn]
• Verfasst von: Yard, Benito A. [VerfasserIn]
• Titel: Altered CD46-mediated T cell co-stimulation in haemodialysis patients
• Verf.angabe: P.-T. Brinkkoetter, S. Marinaki, U. Gottmann, S. Fleckenstein, C. Stump, F.J. Van Der Woude, C. Braun and B.A. Yard
• Jahr: 2005
• Umfang: 8 S.
• Fussnoten: Gesehen am 27.05.2022
• Titel Quelle: Enthalten in: Clinical & experimental immunology
• Ort Quelle: Oxford : Wiley-Blackwell, 1966
• Jahr Quelle: 2005
• Band/Heft Quelle: 139(2005), 3, Seite 534-541
• ISSN Quelle: 1365-2249
• DOI: doi:10.1111/j.1365-2249.2005.02705.x
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Aged
• Sach-SW: Aged, 80 and over
• Sach-SW: Alternative Splicing
• Sach-SW: Analysis of Variance
• Sach-SW: Antigens, CD
• Sach-SW: Case-Control Studies
• Sach-SW: CD4-Positive T-Lymphocytes
• Sach-SW: Cell Proliferation
• Sach-SW: Complement Activation
• Sach-SW: Female
• Sach-SW: Humans
• Sach-SW: Interleukin-10
• Sach-SW: Kidney Failure, Chronic
• Sach-SW: Lymphocyte Activation
• Sach-SW: Male
• Sach-SW: Membrane Cofactor Protein
• Sach-SW: Membrane Glycoproteins
• Sach-SW: Middle Aged
• Sach-SW: Peritoneal Dialysis
• Sach-SW: Polymorphism, Genetic
• Sach-SW: Promoter Regions, Genetic
• Sach-SW: Renal Dialysis
• Sach-SW: Statistics, Nonparametric
• K10plus-PPN: 1804447862

Abstract

While most of our understanding of immune dysfunction in dialysis patients involves alterations in CD28-CD80/86 signalling, nothing is known of CD46-mediated co-stimulation of T cells in these patients. Because C3b/C4b bind to CD46 and complement activation occurs during haemodialysis (HD), we addressed whether CD46-mediated T cell activation is altered in HD (n = 9), peritoneal dialysis (PD) (n = 10) and predialysis patients (n = 8) compared to healthy controls (HC) (n = 8). T cell surface markers, T cell proliferation and interleukin (IL)-10 production were studied in CD4(+)T cells. In addition, CD46 splice-variants and IL-10 promoter gene polymorphisms were studied by reverse transcription (RT) or amplification refractory mutation system-polymerase chain reaction (ARMS-PCR), respectively. In all uraemic patients, irrespective of the stage of renal insufficiency or dialysis modality, a significant increase in the percentage of CD25 positivity in naive CD4(+)T cells was found (64% +/- 21%versus 23% +/- 18%, P < 0.001). Lymphocytes of HD patients proliferated in greater numbers and produced more IL-10 after co-stimulation with anti-CD46 than after co-stimulation with anti-CD28. This was also found in CD4(+)T cells of PD patients, albeit to a lesser extent. In contrast, with T cells of predialysis patients and of HC, co-stimulation via CD28 was more efficient. The observed alterations in T cell proliferation and IL-10 production were associated neither with CD46 splice variants nor with IL-10 promoter gene polymorphisms. Lymphocytes of HD patients show an increased response on CD46 co-stimulation. These data suggest that ongoing complement activation in HD patients may lead to alterations in acquired immunity.