Identifying stem cell numbers and functional heterogeneities during postembryonic organ growth

• Verfasst von: Danciu, Diana-Patricia [VerfasserIn]
• Verfasst von: Stolper, Julian [VerfasserIn]
• Verfasst von: Centanin, Lázaro [VerfasserIn]
• Verfasst von: Marciniak-Czochra, Anna [VerfasserIn]
• Titel: Identifying stem cell numbers and functional heterogeneities during postembryonic organ growth
• Verf.angabe: Diana-Patricia Danciu, Julian Stolper, Lázaro Centanin, and Anna Marciniak-Czochra
• E-Jahr: 2022
• Jahr: February 18, 2022
• Fussnoten: Gesehen am 30.05.2022
• Titel Quelle: Enthalten in: iScience
• Ort Quelle: Amsterdam : Elsevier, 2018
• Jahr Quelle: 2022
• Band/Heft Quelle: 25(2022), 2, Artikel-ID 103819, Seite 1-17
• ISSN Quelle: 2589-0042
• DOI: doi:10.1016/j.isci.2022.103819
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Developmental biology
• Sach-SW: Ichthyology
• Sach-SW: Mathematical biosciences
• K10plus-PPN: 180517553X

Abstract

Uncovering the number of stem cells necessary for organ growth has been challenging in vertebrate systems. Here, we developed a mathematical model characterizing stem cells in the fish gill, an organ displaying non-exhaustive growth. We employ a Markov model, stochastically simulated via an adapted Gillespie algorithm, and further improved through probability theory. The stochastic algorithm produces a simulated dataset for comparison with experimental clonal data by inspecting quantifiable properties. The analytical approach skips the step of artificial data generation and goes directly to the quantification, being more abstract and efficient. We report that a reduced number of stem cells actively contribute to growing and maintaining the gills. The model also highlights a functional heterogeneity among the stem cells involved, where activation and quiescence phases determine their relative growth contribution. Overall, our work presents a method for inferring the number and properties of stem cells required in a lifelong growing system.