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| Verfasst von: | Sattler, Katherine [VerfasserIn]  |
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| | El-Battrawy, Ibrahim [VerfasserIn] |
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| | Cyganek, Lukas [VerfasserIn] |
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| | Lang, Siegfried [VerfasserIn] |
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| | Lan, Huan [VerfasserIn] |
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| | Li, Xin [VerfasserIn] |
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| | Zhao, Zhihan [VerfasserIn] |
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| | Utikal, Jochen [VerfasserIn] |
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| | Wieland, Thomas [VerfasserIn] |
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| | Borggrefe, Martin [VerfasserIn] |
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| | Zhou, Xiao-Bo [VerfasserIn] |
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| | Akın, Ibrahim [VerfasserIn] |
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| Titel: | TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes |
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| Verf.angabe: | Katherine Sattler, Ibrahim El-Battrawy, Lukas Cyganek, Siegfried Lang, Huan Lan, Xin Li, Zhihan Zhao, Jochen Utikal, Thomas Wieland, Martin Borggrefe, Xiaobo Zhou and Ibrahim Akin |
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| E-Jahr: | 2021 |
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| Jahr: | 19 July 2021 |
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| Umfang: | 12 S. |
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| Fussnoten: | Published: 19 July 2021 ; Gesehen am 31.05.2022 |
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| Titel Quelle: | Enthalten in: Scientific reports |
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| Ort Quelle: | [London] : Springer Nature, 2011 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 11(2021), Artikel-ID 14689, Seite 1-12 |
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| ISSN Quelle: | 2045-2322 |
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| Abstract: | The non-selective cation channel transient receptor potential vanilloid 1 (TRPV1) is expressed throughout the cardiovascular system. Recent evidence shows a role for TRPV1 in inflammatory processes. The role of TRPV1 for myocardial inflammation has not been established yet. Human induced pluripotent stem cell (iPSC)-derived cardiomyocytes (hiPSC-CM) from 4 healthy donors were incubated with lipopolysaccharides (LPS, 6 h), TRPV1 agonist capsaicin (CAP, 20 min) or the antagonist capsazepine (CPZ, 20 min). TRPV1 expression was studied by PCR and western blotting. TRPV1 internalization was analyzed by immunofluorescence. Interleukin-6 (IL-6) secretion and phosphorylation of JNK, p38 and ERK were determined by ELISA. TRPV1-associated ion channel current was measured by patch clamp. TRPV1-mRNA and -protein were expressed in hiPSC-CM. TRPV1 was localized in the plasma membrane. LPS significantly increased secretion of IL-6 by 2.3-fold, which was prevented by pre-incubation with CPZ. LPS induced TRPV1 internalization. Phosphorylation levels of ERK, p38 or JNK were not altered by TRPV1 stimulation or inhibition. LPS and IL-6 significantly lowered TRPV1-mediated ion channel current. TRPV1 mediates the LPS-induced inflammation in cardiomyocytes, associated with changes of cellular electrophysiology. LPS-induced inflammation results in TRPV1 internalization. Further studies have to examine the underlying pathways and the clinical relevance of these findings. |
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| DOI: | doi:10.1038/s41598-021-93958-3 |
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| URL: | kostenfrei: Volltext: https://doi.org/10.1038/s41598-021-93958-3 |
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| | kostenfrei: Volltext: https://www.nature.com/articles/s41598-021-93958-3 |
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| | DOI: https://doi.org/10.1038/s41598-021-93958-3 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Cardiovascular biology |
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| | Mechanisms of disease |
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| K10plus-PPN: | 180534854X |
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| Verknüpfungen: | → Zeitschrift |
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| Lokale URL UB: |  Zum Volltext |
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TRPV1 activation and internalization is part of the LPS-induced inflammation in human iPSC-derived cardiomyocytes / Sattler, Katherine [VerfasserIn]; 19 July 2021 (Online-Ressource)
