Expression profile of human renal mesangial cells is altered by infection with pathogenic Puumala orthohantavirus

• Verfasst von: Nußhag, Christian [VerfasserIn]
• Verfasst von: Boegelein, Lukas [VerfasserIn]
• Verfasst von: Schreiber, Pamela [VerfasserIn]
• Verfasst von: Essbauer, Sandra [VerfasserIn]
• Verfasst von: Osberghaus, Anja [VerfasserIn]
• Verfasst von: Zeier, Martin [VerfasserIn]
• Verfasst von: Krautkrämer, Ellen [VerfasserIn]
• Titel: Expression profile of human renal mesangial cells is altered by infection with pathogenic Puumala orthohantavirus
• Verf.angabe: Christian Nusshag, Lukas Boegelein, Pamela Schreiber, Sandra Essbauer, Anja Osberghaus, Martin Zeier and Ellen Krautkrämer
• E-Jahr: 2022
• Jahr: 15 April 2022
• Umfang: 16 S.
• Fussnoten: This article belongs to the Section "Human Virology and Viral Diseases" ; Gesehen am 01.06.2022
• Titel Quelle: Enthalten in: Viruses
• Ort Quelle: Basel : MDPI, 2009
• Jahr Quelle: 2022
• Band/Heft Quelle: 14(2022), 4 vom: Apr., Artikel-ID 823, Seite 1-16
• ISSN Quelle: 1999-4915
• DOI: doi:10.3390/v14040823
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: acute kidney injury
• Sach-SW: glomerulus
• Sach-SW: kidney
• Sach-SW: mesangial cell
• Sach-SW: orthohantavirus
• Sach-SW: proteome
• K10plus-PPN: 1805507605

Abstract

Acute kidney injury (AKI) with proteinuria is a hallmark of infections with Eurasian orthohantaviruses. Different kidney cells are identified as target cells of hantaviruses. Mesangial cells may play a central role in the pathogenesis of AKI by regulation of inflammatory mediators and signaling cascades. Therefore, we examined the characteristics of hantavirus infection on human renal mesangial cells (HRMCs). Receptor expression and infection with pathogenic Puumala virus (PUUV) and low-pathogenic Tula virus (TULV) were explored. To analyze changes in protein expression in infected mesangial cells, we performed a proteome profiler assay analyzing 38 markers of kidney damage. We compared the proteome profile of in vitro-infected HRMCs with the profile detected in urine samples of 11 patients with acute hantavirus infection. We observed effective productive infection of HRMCs with pathogenic PUUV, but only poor abortive infection for low-pathogenic TULV. PUUV infection resulted in the deregulation of proteases, adhesion proteins, and cytokines associated with renal damage. The urinary proteome profile of hantavirus patients demonstrated also massive changes, which in part correspond to the alterations observed in the in vitro infection of HRMCs. The direct infection of mesangial cells may induce a local environment of signal mediators that contributes to AKI in hantavirus infection.