Adipogenic differentiation of human mesenchymal stromal cells is down-regulated by microRNA-369-5p and up-regulated by microRNA-371

• Verfasst von: Bork, Simone [VerfasserIn]
• Verfasst von: Horn, Patrick [VerfasserIn]
• Verfasst von: Castoldi, Mirco [VerfasserIn]
• Verfasst von: Hellwig, Isabelle [VerfasserIn]
• Verfasst von: Ho, Anthony Dick [VerfasserIn]
• Verfasst von: Wagner, Wolfgang [VerfasserIn]
• Titel: Adipogenic differentiation of human mesenchymal stromal cells is down-regulated by microRNA-369-5p and up-regulated by microRNA-371
• Verf.angabe: Simone Bork, Patrick Horn, Mirco Castoldi, Isabelle Hellwig, Anthony D. Ho, and Wolfgang Wagner
• Jahr: 2011
• Umfang: 9 S.
• Fussnoten: First published: 06 December 2010 ; Gesehen am 02.06.2022
• Titel Quelle: Enthalten in: Journal of cellular physiology
• Ort Quelle: New York, NY [u.a.] : Wiley-Liss, 1932
• Jahr Quelle: 2011
• Band/Heft Quelle: 226(2011), 9, Seite 2226-2234
• ISSN Quelle: 1097-4652
• DOI: doi:10.1002/jcp.22557
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1805741985

Abstract

Long-term culture of human mesenchymal stromal cells (MSC) has implications on their proliferation and differentiation potential and we have demonstrated that this is associated with up-regulation of the five microRNAs miR-29c, miR-369-5p, miR-371, miR-499, and let-7f. In this study, we examined the role of these senescence-associated microRNAs for cellular aging and differentiation of MSC. Proliferation was reduced upon transfection with miR-369-5p, miR-371, and miR-499. Adipogenic differentiation was impaired by miR-369-5p whereas it was highly increased by miR-371. This was accompanied by respective gene expression changes of some adipogenic key molecules (adiponectin and fatty acid-binding protein 4 [FABP4]). Furthermore luciferase reporter assay indicated that FABP4 is a direct target of miR-369-5p. Microarray analysis upon adipogenic or osteogenic differentiation revealed down-regulation of several microRNAs albeit miR-369-5p and miR-371 were not affected. Expression of the de novo DNA methyltransferases DNMT3A and DNMT3B was up-regulated by transfection of miR-371 whereas expression of DNMT3A was down-regulated by miR-369-5p. In summary, we identified miR-369-5p and miR-371 as antagonistic up-stream regulators of adipogenic differentiation and this might be indirectly mediated by epigenetic modifications. J. Cell. Physiol. 226: 2226-2234, 2011. © 2010 Wiley-Liss, Inc.