| Online-Ressource |
Verfasst von: | Bork, Simone [VerfasserIn]  |
| Horn, Patrick [VerfasserIn]  |
| Castoldi, Mirco [VerfasserIn]  |
| Hellwig, Isabelle [VerfasserIn]  |
| Ho, Anthony Dick [VerfasserIn]  |
| Wagner, Wolfgang [VerfasserIn]  |
Titel: | Adipogenic differentiation of human mesenchymal stromal cells is down-regulated by microRNA-369-5p and up-regulated by microRNA-371 |
Verf.angabe: | Simone Bork, Patrick Horn, Mirco Castoldi, Isabelle Hellwig, Anthony D. Ho, and Wolfgang Wagner |
Jahr: | 2011 |
Umfang: | 9 S. |
Fussnoten: | First published: 06 December 2010 ; Gesehen am 02.06.2022 |
Titel Quelle: | Enthalten in: Journal of cellular physiology |
Ort Quelle: | New York, NY [u.a.] : Wiley-Liss, 1932 |
Jahr Quelle: | 2011 |
Band/Heft Quelle: | 226(2011), 9, Seite 2226-2234 |
ISSN Quelle: | 1097-4652 |
Abstract: | Long-term culture of human mesenchymal stromal cells (MSC) has implications on their proliferation and differentiation potential and we have demonstrated that this is associated with up-regulation of the five microRNAs miR-29c, miR-369-5p, miR-371, miR-499, and let-7f. In this study, we examined the role of these senescence-associated microRNAs for cellular aging and differentiation of MSC. Proliferation was reduced upon transfection with miR-369-5p, miR-371, and miR-499. Adipogenic differentiation was impaired by miR-369-5p whereas it was highly increased by miR-371. This was accompanied by respective gene expression changes of some adipogenic key molecules (adiponectin and fatty acid-binding protein 4 [FABP4]). Furthermore luciferase reporter assay indicated that FABP4 is a direct target of miR-369-5p. Microarray analysis upon adipogenic or osteogenic differentiation revealed down-regulation of several microRNAs albeit miR-369-5p and miR-371 were not affected. Expression of the de novo DNA methyltransferases DNMT3A and DNMT3B was up-regulated by transfection of miR-371 whereas expression of DNMT3A was down-regulated by miR-369-5p. In summary, we identified miR-369-5p and miR-371 as antagonistic up-stream regulators of adipogenic differentiation and this might be indirectly mediated by epigenetic modifications. J. Cell. Physiol. 226: 2226-2234, 2011. © 2010 Wiley-Liss, Inc. |
DOI: | doi:10.1002/jcp.22557 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1002/jcp.22557 |
| Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.22557 |
| DOI: https://doi.org/10.1002/jcp.22557 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1805741985 |
Verknüpfungen: | → Zeitschrift |
Adipogenic differentiation of human mesenchymal stromal cells is down-regulated by microRNA-369-5p and up-regulated by microRNA-371 / Bork, Simone [VerfasserIn]; 2011 (Online-Ressource)