Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Bork, Simone [VerfasserIn]   i
 Horn, Patrick [VerfasserIn]   i
 Castoldi, Mirco [VerfasserIn]   i
 Hellwig, Isabelle [VerfasserIn]   i
 Ho, Anthony Dick [VerfasserIn]   i
 Wagner, Wolfgang [VerfasserIn]   i
Titel:Adipogenic differentiation of human mesenchymal stromal cells is down-regulated by microRNA-369-5p and up-regulated by microRNA-371
Verf.angabe:Simone Bork, Patrick Horn, Mirco Castoldi, Isabelle Hellwig, Anthony D. Ho, and Wolfgang Wagner
Jahr:2011
Umfang:9 S.
Fussnoten:First published: 06 December 2010 ; Gesehen am 02.06.2022
Titel Quelle:Enthalten in: Journal of cellular physiology
Ort Quelle:New York, NY [u.a.] : Wiley-Liss, 1932
Jahr Quelle:2011
Band/Heft Quelle:226(2011), 9, Seite 2226-2234
ISSN Quelle:1097-4652
Abstract:Long-term culture of human mesenchymal stromal cells (MSC) has implications on their proliferation and differentiation potential and we have demonstrated that this is associated with up-regulation of the five microRNAs miR-29c, miR-369-5p, miR-371, miR-499, and let-7f. In this study, we examined the role of these senescence-associated microRNAs for cellular aging and differentiation of MSC. Proliferation was reduced upon transfection with miR-369-5p, miR-371, and miR-499. Adipogenic differentiation was impaired by miR-369-5p whereas it was highly increased by miR-371. This was accompanied by respective gene expression changes of some adipogenic key molecules (adiponectin and fatty acid-binding protein 4 [FABP4]). Furthermore luciferase reporter assay indicated that FABP4 is a direct target of miR-369-5p. Microarray analysis upon adipogenic or osteogenic differentiation revealed down-regulation of several microRNAs albeit miR-369-5p and miR-371 were not affected. Expression of the de novo DNA methyltransferases DNMT3A and DNMT3B was up-regulated by transfection of miR-371 whereas expression of DNMT3A was down-regulated by miR-369-5p. In summary, we identified miR-369-5p and miR-371 as antagonistic up-stream regulators of adipogenic differentiation and this might be indirectly mediated by epigenetic modifications. J. Cell. Physiol. 226: 2226-2234, 2011. © 2010 Wiley-Liss, Inc.
DOI:doi:10.1002/jcp.22557
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1002/jcp.22557
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.22557
 DOI: https://doi.org/10.1002/jcp.22557
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1805741985
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68926187   QR-Code
zum Seitenanfang