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| Verfasst von: | Salg, Gabriel Alexander [VerfasserIn]  |
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| | Zillich, Eric [VerfasserIn] |
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| | Neulinger-Muñoz, Matthias [VerfasserIn] |
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| | Gerhardus, Jamina [VerfasserIn] |
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| | Cebulla, Daniel [VerfasserIn] |
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| | Bludszuweit-Philipp, Catrin [VerfasserIn] |
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| | Vieira, Vitor Manuel Machado [VerfasserIn] |
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| | Nickel, Felix [VerfasserIn] |
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| | Herr, Ingrid [VerfasserIn] |
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| | Blaeser, Andreas [VerfasserIn] |
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| | Giese, Nathalia [VerfasserIn] |
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| | Hackert, Thilo [VerfasserIn] |
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| | Kenngott, Hannes Götz [VerfasserIn] |
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| Titel: | Toward 3D-bioprinting of an endocrine pancreas |
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| Titelzusatz: | a building-block concept for bioartificial insulin-secreting tissue |
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| Verf.angabe: | Gabriel A Salg, Eric Poisel, Matthias Neulinger-Munoz, Jamina Gerhardus, Daniel Cebulla, Catrin Bludszuweit-Philipp, Vitor Vieira, Felix Nickel, Ingrid Herr, Andreas Blaeser, Nathalia A Giese, Thilo Hackert and Hannes G Kenngott |
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| Jahr: | 2022 |
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| Umfang: | 19 S. |
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| Fussnoten: | Gesehen am 02.06.2022 |
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| Titel Quelle: | Enthalten in: Journal of tissue engineering |
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| Ort Quelle: | London : Sage, 2010 |
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| Jahr Quelle: | 2022 |
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| Band/Heft Quelle: | 13(2022), Artikel-ID 20417314221091033, Seite 1-19 |
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| ISSN Quelle: | 2041-7314 |
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| Abstract: | Three-dimensional bioprinting of an endocrine pancreas is a promising future curative treatment for patients with insulin secretion deficiency. In this study, we present an end-to-end concept from the molecular to the macroscopic level. Building-blocks for a hybrid scaffold device of hydrogel and functionalized polycaprolactone were manufactured by 3D-(bio)printing. Pseudoislet formation from INS-1 cells after bioprinting resulted in a viable and proliferative experimental model. Transcriptomics showed an upregulation of proliferative and ß-cell-specific signaling cascades, downregulation of apoptotic pathways, overexpression of extracellular matrix proteins, and VEGF induced by pseudoislet formation and 3D-culture. Co-culture with endothelial cells created a natural cellular niche with enhanced insulin secretion after glucose stimulation. Survival and function of pseudoislets after explantation and extensive scaffold vascularization of both hydrogel and heparinized polycaprolactone were demonstrated in vivo. Computer simulations of oxygen, glucose and insulin flows were used to evaluate scaffold architectures and Langerhans islets at a future perivascular transplantation site. |
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| DOI: | doi:10.1177/20417314221091033 |
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| URL: |  ...Bitte verwenden Sie für den Volltextzugang die unten stehende lokale URL.
kostenfrei: Volltext: https://doi.org/10.1177/20417314221091033 |
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| | kostenfrei: Volltext: https://journals.sagepub.com/doi/10.1177/20417314221091033 |
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| | DOI: https://doi.org/10.1177/20417314221091033 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Bioprinting |
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| | diabetes |
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| | endocrine pancreas |
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| | next-generation sequencing |
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| | tissue engineering |
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| K10plus-PPN: | 1805814192 |
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| Verknüpfungen: | → Zeitschrift |
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| Lokale URL UB: |  Zum Volltext |
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