| Online-Ressource |
Verfasst von: | Brauckhoff, Antje [VerfasserIn]  |
| Malz, Mona [VerfasserIn]  |
| Tschaharganeh, Darjus-Felix [VerfasserIn]  |
| Malek, Nisar Peter [VerfasserIn]  |
| Weber, Achim [VerfasserIn]  |
| Riener, Marc-Oliver [VerfasserIn]  |
| Soll, Christopher [VerfasserIn]  |
| Samarin, Jana [VerfasserIn]  |
| Bissinger, Michaela [VerfasserIn]  |
| Schmidt, Jan [VerfasserIn]  |
| Longerich, Thomas [VerfasserIn]  |
| Ehemann, Volker [VerfasserIn]  |
| Schirmacher, Peter [VerfasserIn]  |
| Breuhahn, Kai [VerfasserIn]  |
Titel: | Nuclear expression of the ubiquitin ligase seven in absentia homolog (SIAH)-1 induces proliferation and migration of liver cancer cells |
Verf.angabe: | Antje Brauckhoff, Mona Malz, Darjus Tschaharganeh, Nisar Malek, Achim Weber, Marc-Oliver Riener, Christopher Soll, Jana Samarin, Michaela Bissinger, Jan Schmidt, Thomas Longerich, Volker Ehemann, Peter Schirmacher, Kai Breuhahn |
E-Jahr: | 2011 |
Jahr: | 26 February 2011 |
Umfang: | 9 S. |
Fussnoten: | Gesehen am 03.06.2022 |
Titel Quelle: | Enthalten in: Journal of hepatology |
Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1985 |
Jahr Quelle: | 2011 |
Band/Heft Quelle: | 55(2011), 5, Seite 1049-1057 |
ISSN Quelle: | 1600-0641 |
Abstract: | Background & Aims - Differential expression of tumor-relevant proteins based on aberrant proteasomal degradation may contribute to human (hepato)carcinogenesis. Recently, we identified the E3 ubiquitin ligase seven in absentia homolog (SIAH)-1 as frequently dysregulated in human hepatocellular carcinoma (HCC). We therefore systematically analyzed the expression, functional relevance, as well as possible downstream effectors of SIAH-1 in human liver carcinogenesis. - Methods - SIAH-1 expression was analyzed at the transcript and protein levels in human hepatocarcinogenesis and in HCC cells. Proliferation, apoptosis, and migration of different HCC cell lines were examined after siRNA-mediated inhibition of SIAH-1. In order to identify downstream effectors that mediate SIAH-1 effects, correlative analyses of protein expression profiles were performed. - Results - In HCC tissues both reduction of cytoplasmic SIAH-1 and especially its nuclear accumulation positively correlated with HCC progression. RNA interference revealed that nuclear expression of SIAH-1 predominantly supported HCC cell proliferation and migration while only moderately affecting anti-apoptosis. In de-differentiated human HCCs, nuclear SIAH-1 accumulation significantly correlated with the expression of the transcription factor far-upstream element (FUSE)-binding protein (FBP)-3. In vitro, SIAH-1 positively and indirectly regulated FBP-3 which itself primarily supported HCC cell proliferation. Indeed, high level expression of FBP-3 in human HCCs significantly correlated with reduced overall survival of patients. - Conclusions - Nuclear accumulation of the E3 ubiquitin ligase SIAH-1 supports different pro-tumorigenic cellular processes associated with tumor growth and tumor cell dissemination in human hepatocarcinogenesis. It promotes HCC cell proliferation by at least partly employing the transcription factor FBP-3. Therefore, interference with SIAH-1 activity represents a promising approach to suppress HCC growth. |
DOI: | doi:10.1016/j.jhep.2011.02.019 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.jhep.2011.02.019 |
| Volltext: https://www.sciencedirect.com/science/article/pii/S0168827811002042 |
| DOI: https://doi.org/10.1016/j.jhep.2011.02.019 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Bibliogr. Hinweis: | Erscheint auch als : Druck-Ausgabe: Brauckhoff, Antje: Nuclear expression of the ubiquitin ligase seven in absentia homolog (SIAH)-1 induces proliferation and migration of liver cancer cells. - 2011 |
Sach-SW: | Far-upstream element binding protein |
| FBP-3 |
| HCC |
| Hepatocellular carcinoma |
| Proteasome |
| Survival |
| Transcription factor |
K10plus-PPN: | 180592043X |
Verknüpfungen: | → Zeitschrift |
Nuclear expression of the ubiquitin ligase seven in absentia homolog (SIAH)-1 induces proliferation and migration of liver cancer cells / Brauckhoff, Antje [VerfasserIn]; 26 February 2011 (Online-Ressource)