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| Verfasst von: | Neuvians, Tanja Pascale [VerfasserIn]  |
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| | Gashaw, Isabella [VerfasserIn] |
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| | Sauer, Christian [VerfasserIn] |
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| | Ostau, Christian Eike von [VerfasserIn] |
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| | Kliesch, Sabine [VerfasserIn] |
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| | Bergmann, Martin [VerfasserIn] |
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| | Häcker, Axel [VerfasserIn] |
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| | Grobholz, Rainer [VerfasserIn] |
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| Titel: | Standardization strategy for quantitative PCR in human seminoma and normal testis |
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| Verf.angabe: | Tanja Pascale Neuvians, Isabella Gashaw, Christian Georg Sauer, Christian von Ostau, Sabine Kliesch, Martin Bergmann, Axel Häcker, Rainer Grobholz |
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| E-Jahr: | 2005 |
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| Jahr: | [4 May 2005] |
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| Umfang: | 9 S. |
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| Illustrationen: | Diagramme |
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| Fussnoten: | Gesehen am 08.06.2022 |
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| Titel Quelle: | Enthalten in: Journal of biotechnology |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1984 |
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| Jahr Quelle: | 2005 |
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| Band/Heft Quelle: | 117(2005), 2 vom: Mai, Seite 163-171 |
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| ISSN Quelle: | 1873-4863 |
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| Abstract: | Housekeeping genes are commonly used as endogenous references in quantitative RT-PCR. Ideally these genes are constitutionally expressed by all cell types and do not vary under experimental conditions. Tissues of 9 normal testes and 22 classical pure seminoma were obtained for RNA-extraction. Real-time RT-PCR was used to examine the mRNA-expression of ubiquitin C, beta-actin, GAPDH, 18S ribosomal RNA (18S rRNA) and porphobilinogen-deaminase (PBGD). Additionally, 3 normal testicular tissues and 39 seminoma, including 1 normal testis and 17 seminoma of the RT-PCR group, were utilized for microarray analyses. Ubiquitin C (protein degradation) was down-regulated, GAPDH (carbohydrate metabolism), beta-actin (cytoskeleton), 18S rRNA (ribosome) and PBGD (porphyrin metabolism) were up-regulated in seminoma. A normalization of the target gene data with up-regulated housekeeping genes would equalize or underestimate up-regulated data and overestimate down-regulated data. We demonstrate that none of the investigated housekeeping genes is suitable for normalization of the target gene RT-PCR data, but may be essential for tumor metabolism in human seminoma. Further, we developed a standardization strategy, which is applicable to many experimental investigations. |
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| DOI: | doi:10.1016/j.jbiotec.2005.01.011 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.jbiotec.2005.01.011 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S0168165605000568 |
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| | DOI: https://doi.org/10.1016/j.jbiotec.2005.01.011 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Beta-actin |
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| | GAPDH |
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| | Housekeeping gene |
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| | Quantitative PCR |
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| | Seminoma |
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| | Testis |
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| K10plus-PPN: | 180623727X |
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| Verknüpfungen: | → Zeitschrift |
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Standardization strategy for quantitative PCR in human seminoma and normal testis / Neuvians, Tanja Pascale [VerfasserIn]; [4 May 2005] (Online-Ressource)
