Bax, Bcl-2, Fas and Fas-L antigen expression in human seminoma

• Verfasst von: Grobholz, Rainer [VerfasserIn]
• Verfasst von: Zentgraf, Hanswalter [VerfasserIn]
• Verfasst von: Köhrmann, Kai-Uwe [VerfasserIn]
• Verfasst von: Bleyl, Uwe [VerfasserIn]
• Titel: Bax, Bcl-2, Fas and Fas-L antigen expression in human seminoma
• Titelzusatz: correlation with the apoptotic index
• Verf.angabe: Rainer Grobholz, Hanswalter Zentgraf, Kai-Uwe Köhrmann and Uwe Bleyl
• E-Jahr: 2003
• Jahr: 14 February 2003
• Jahr des Originals: 2002
• Umfang: 9 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 08.06.2022
• Titel Quelle: Enthalten in: Acta pathologica, microbiologica et immunologica Scandinavica
• Ort Quelle: Oxford : Wiley-Blackwell, 1988
• Jahr Quelle: 2002
• Band/Heft Quelle: 110(2002), 10 vom: Okt., Seite 724-732
• ISSN Quelle: 1600-0463
• DOI: doi:10.1034/j.1600-0463.2002.1101006.x
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: apoptosis
• Sach-SW: Bax
• Sach-SW: Bcl-2
• Sach-SW: Fas
• Sach-SW: Fas-L.
• Sach-SW: Seminoma
• K10plus-PPN: 1806240831

Abstract

Apoptosis plays a crucial role in the regulation of spermatogenesis in male germ cells and is, at least in part, modulated by Bcl-2, Bax, and the Fas pathway. Seminomas have a favourable outcome and respond to radio-/chemotherapy with an increased rate of apoptosis. The expression of Bax, Bcl-2, Fas and Fas-ligand (Fas-L) in human seminoma was evaluated and correlated with the apoptotic index. Twenty-nine classical seminomas were examined by immunohistochemistry and Western blotting using antibodies against Bax, Bcl-2, Fas and Fas-L. Apoptosis was detected by in-situ end-labeling of fragmented DNA and the apoptotic index (AI) was determined. Expression of Fas was found in 26 (89.7%) of Fas-L in 24 seminomas (82.2%); none of the tumours expressed Bcl-2. No correlation between the AI and Fas, Fas-L or Bcl-2 expression was found. Bax was demonstrated in 20/29 tumours (69%). Bax-positive tumours showed an increased AI of 4.75±2.38% in contrast to 2.60±1.23% of the Bax-negative tumours (P=0.002). The number of Bax-positive tumour cells and apoptotic cells revealed a significant correlation using χ2-test (P=0.04) and linear regression (r=0.54, P=0.001). Therefore, Bax seems to play a determinant role in the modulation of apoptosis in human seminoma that may be linked to a favourable outcome.