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Verfasst von:Muranyi, Walter [VerfasserIn]   i
 Schwerk, Christian [VerfasserIn]   i
 Herold, Rosanna [VerfasserIn]   i
 Stump-Guthier, Carolin [VerfasserIn]   i
 Lampe, Marko [VerfasserIn]   i
 Fallier-Becker, Petra [VerfasserIn]   i
 Weiß, Christel [VerfasserIn]   i
 Sticht, Carsten [VerfasserIn]   i
 Ishikawa, Hiroshi [VerfasserIn]   i
 Schroten, Horst [VerfasserIn]   i
Titel:Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell type in vitro model of the choroid plexus
Verf.angabe:Walter Muranyi, Christian Schwerk, Rosanna Herold, Carolin Stump-Guthier, Marko Lampe, Petra Fallier-Becker, Christel Weiß, Carsten Sticht, Hiroshi Ishikawa, and Horst Schroten
E-Jahr:2022
Jahr:June 17, 2022
Umfang:23 S.
Fussnoten:Gesehen am 10.06.2022
Titel Quelle:Enthalten in: iScience
Ort Quelle:Amsterdam : Elsevier, 2018
Jahr Quelle:2022
Band/Heft Quelle:25(2022), 6, Artikel-ID 104383, Seite 1-23
ISSN Quelle:2589-0042
Abstract:The choroid plexus (CP) is a highly vascularized structure containing endothelial and epithelial cells located in the ventricular system of the central nervous system (CNS). The role of the fenestrated CP endothelium is under-researched and requires the generation of an immortalized CP endothelial cell line with preserved features. Transduction of primary human CP endothelial cells (HCPEnC) with the human telomerase reverse transcriptase (hTERT) resulted in immortalized HCPEnC (iHCPEnC), which grew as monolayer with contact inhibition, formed capillary-like tubes in Matrigel, and showed no colony growth in soft agar. iHCPEnC expressed pan-endothelial markers and presented characteristic plasmalemma vesicle-associated protein-containing structures. Cultivation of iHCPEnC and human epithelial CP papilloma (HIBCPP) cells on opposite sides of cell culture filter inserts generated an in vitro model with a consistently enhanced barrier function specifically by iHCPEnC. Overall, iHCPEnC present a tool that will contribute to the understanding of CP organ functions, especially endothelial-epithelial interplay.
DOI:doi:10.1016/j.isci.2022.104383
URL:kostenfrei: Volltext: https://doi.org/10.1016/j.isci.2022.104383
 kostenfrei: Volltext: https://www.sciencedirect.com/science/article/pii/S258900422200654X
 DOI: https://doi.org/10.1016/j.isci.2022.104383
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Biological sciences
 Biological sciences research methodologies
 Biology experimental methods
 Cell biology
K10plus-PPN:1806761793
Verknüpfungen:→ Zeitschrift
 
 
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