| |  Online-Ressource |
|---|
| Verfasst von: | Williams, Jessica [VerfasserIn]  |
|---|
| | Ingham, Victoria [VerfasserIn] |
|---|
| | Morris, Marion [VerfasserIn] |
|---|
| | Toé, Kobié Hyacinthe [VerfasserIn] |
|---|
| | Hien, Aristide S. [VerfasserIn] |
|---|
| | Morgan, John C. [VerfasserIn] |
|---|
| | Dabiré, Roch K. [VerfasserIn] |
|---|
| | Guelbéogo, Wamdagogo Moussa [VerfasserIn] |
|---|
| | Sagnon, N’Falé [VerfasserIn] |
|---|
| | Ranson, Hilary [VerfasserIn] |
|---|
| Titel: | Sympatric populations of the anopheles gambiae complex in Southwest Burkina Faso evolve multiple diverse resistance mechanisms in response to intense selection pressure with pyrethroids |
|---|
| Verf.angabe: | Jessica Williams, Victoria A. Ingham, Marion Morris, Kobié Hyacinthe Toé, Aristide S. Hien, John C. Morgan, Roch K. Dabiré, Wamdagogo Moussa Guelbéogo, N’Falé Sagnon and Hilary Ranson |
|---|
| E-Jahr: | 2022 |
|---|
| Jahr: | 28 February 2022 |
|---|
| Umfang: | 20 S. |
|---|
| Fussnoten: | Gesehen am 11.06.2022 |
|---|
| Titel Quelle: | Enthalten in: Insects |
|---|
| Ort Quelle: | Basel : MDPI, 2010 |
|---|
| Jahr Quelle: | 2022 |
|---|
| Band/Heft Quelle: | 13(2022), 3 vom: März, Artikel-ID 247, Seite 1-20 |
|---|
| ISSN Quelle: | 2075-4450 |
|---|
| Abstract: | Pyrethroid resistance in the Anopheles vectors of malaria is driving an urgent search for new insecticides that can be used in proven vector control tools such as insecticide treated nets (ITNs). Screening for potential new insecticides requires access to stable colonies of the predominant vector species that contain the major pyrethroid resistance mechanisms circulating in wild populations. Southwest Burkina Faso is an apparent hotspot for the emergence of pyrethroid resistance in species of the Anopheles gambiae complex. We established stable colonies from larval collections across this region and characterised the resistance phenotype and underpinning genetic mechanisms. Three additional colonies were successfully established (1 An. coluzzii, 1 An. gambiae and 1 An. arabiensis) to add to the 2 An. coluzzii colonies already established from this region; all 5 strains are highly resistant to pyrethroids. Synergism assays found that piperonyl butoxide (PBO) exposure was unable to fully restore susceptibility although exposure to a commercial ITN containing PBO resulted in 100% mortality. All colonies contained resistant alleles of the voltage gated sodium channel but with differing proportions of alternative resistant haplotypes. RNAseq data confirmed the role of P450s, with CYP6P3 and CYP6Z2 elevated in all 5 strains, and identified many other resistance mechanisms, some found across strains, others unique to a particular species. These strains represent an important resource for insecticide discovery and provide further insights into the complex genetic changes driving pyrethroid resistance. |
|---|
| DOI: | doi:10.3390/insects13030247 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3390/insects13030247 |
|---|
| | Volltext: https://www.mdpi.com/2075-4450/13/3/247 |
|---|
| | DOI: https://doi.org/10.3390/insects13030247 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | cuticular resistance |
|---|
| | cytochrome P450s |
|---|
| | insecticide resistance |
|---|
| | insecticide treated nets |
|---|
| | kdr |
|---|
| | malaria vector |
|---|
| K10plus-PPN: | 1806817888 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Sympatric populations of the anopheles gambiae complex in Southwest Burkina Faso evolve multiple diverse resistance mechanisms in response to intense selection pressure with pyrethroids / Williams, Jessica [VerfasserIn]; 28 February 2022 (Online-Ressource)
