PAF-Receptor in inflammatory versus non inflammatory human epidermis, cell cultures and embryonal cells

• Verfasst von: Bayerl, Christiane [VerfasserIn]
• Verfasst von: Brandt, H. [VerfasserIn]
• Verfasst von: Niemczyk, M. [VerfasserIn]
• Verfasst von: Müller-Decker, Karin [VerfasserIn]
• Verfasst von: Gretz, Norbert [VerfasserIn]
• Titel: PAF-Receptor in inflammatory versus non inflammatory human epidermis, cell cultures and embryonal cells
• Verf.angabe: C. Bayerl, H. Brandt, M. Niemczyk, K. Müller-Decker and N. Gretz
• E-Jahr: 2003
• Jahr: June 2003
• Umfang: 4 S.
• Illustrationen: Illustrationen
• Fussnoten: Gesehen am 22.06.2022
• Titel Quelle: Enthalten in: Inflammation research
• Ort Quelle: Cham : Springer International Publishing AG, 1969
• Jahr Quelle: 2003
• Band/Heft Quelle: 52(2003), 7 vom: Juni, Seite 283-286
• ISSN Quelle: 1420-908X
• DOI: doi:10.1007/s00011-003-1172-x
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Key words:PAF (platelet activating factor)-receptor - Keratinocytes - Fibroblasts - Immunohistochemistry - Western-blot
• K10plus-PPN: 1807478203

Abstract

Objective: PAF-R (platelet activating factor-receptor) has been found on human keratinocytes to bind PAF, a proinflammatory phospholipid. We aimed to study PAF-R in a range of dermal cell lines and in samples of normal and psoriatic human skin to learn about its further role in humans.Methods: PAF-R was labeled immunocytochemically, histochemically and additionally studied with western blotting in human keratinocytes, human fibroblasts, embryonal keratinocytes, tumor cell lines and samples of normal and psoriatic human skin.Results: Keratinocytes from adult and embryonal epidermis of the 20th week of pregnancy showed a low level of labeling for PAF-R, but 3 ± 0.05% of plantar keratinocytes from adults were positive as were 4.2 ± 0.05% of embryonal plantar keratinocytes from the 21st week of pregnancy. In fibroblasts from adult and embryonal epidermis the protein was expressed at low levels. Western blotting revealed PAF-R positive bands at 67 k.Da in normal human skin and psoriasis, in psoriasis additionally at 45 k.Da. A 68 k.Da band was found in the colon cancer line HT 29 (control), and HaCaT cells, in embryonal keratinocytes additionally at 116 k.Da.Conclusions: PAF-R seems not to be important for embryonal or adult fibroblasts. In embryonal keratinocytes it is turned on after the 21st pregnancy week in a few cells seen as a band of 67 k.Da and at 116 k.Da, the latter is not found in adult keratinocytes. An additional 45 k.Da band of PAF-R was found in psoriasis that might stand for a truncated receptor. In the epithelial tumor cell line HaCaT and the HT29 colon cancer cell line PAF-R characterizes the anti-apoptotic effect of this receptor propagating tumor proliferation.