Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Bayerl, Christiane [VerfasserIn]  |
| Brandt, H. [VerfasserIn]  |
| Niemczyk, M. [VerfasserIn]  |
| Müller-Decker, Karin [VerfasserIn]  |
| Gretz, Norbert [VerfasserIn]  |
Titel: | PAF-Receptor in inflammatory versus non inflammatory human epidermis, cell cultures and embryonal cells |
Verf.angabe: | C. Bayerl, H. Brandt, M. Niemczyk, K. Müller-Decker and N. Gretz |
E-Jahr: | 2003 |
Jahr: | June 2003 |
Umfang: | 4 S. |
Illustrationen: | Illustrationen |
Fussnoten: | Gesehen am 22.06.2022 |
Titel Quelle: | Enthalten in: Inflammation research |
Ort Quelle: | Cham : Springer International Publishing AG, 1969 |
Jahr Quelle: | 2003 |
Band/Heft Quelle: | 52(2003), 7 vom: Juni, Seite 283-286 |
ISSN Quelle: | 1420-908X |
Abstract: | Objective: PAF-R (platelet activating factor-receptor) has been found on human keratinocytes to bind PAF, a proinflammatory phospholipid. We aimed to study PAF-R in a range of dermal cell lines and in samples of normal and psoriatic human skin to learn about its further role in humans.Methods: PAF-R was labeled immunocytochemically, histochemically and additionally studied with western blotting in human keratinocytes, human fibroblasts, embryonal keratinocytes, tumor cell lines and samples of normal and psoriatic human skin.Results: Keratinocytes from adult and embryonal epidermis of the 20th week of pregnancy showed a low level of labeling for PAF-R, but 3 ± 0.05% of plantar keratinocytes from adults were positive as were 4.2 ± 0.05% of embryonal plantar keratinocytes from the 21st week of pregnancy. In fibroblasts from adult and embryonal epidermis the protein was expressed at low levels. Western blotting revealed PAF-R positive bands at 67 k.Da in normal human skin and psoriasis, in psoriasis additionally at 45 k.Da. A 68 k.Da band was found in the colon cancer line HT 29 (control), and HaCaT cells, in embryonal keratinocytes additionally at 116 k.Da.Conclusions: PAF-R seems not to be important for embryonal or adult fibroblasts. In embryonal keratinocytes it is turned on after the 21st pregnancy week in a few cells seen as a band of 67 k.Da and at 116 k.Da, the latter is not found in adult keratinocytes. An additional 45 k.Da band of PAF-R was found in psoriasis that might stand for a truncated receptor. In the epithelial tumor cell line HaCaT and the HT29 colon cancer cell line PAF-R characterizes the anti-apoptotic effect of this receptor propagating tumor proliferation. |
DOI: | doi:10.1007/s00011-003-1172-x |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1007/s00011-003-1172-x |
| DOI: https://doi.org/10.1007/s00011-003-1172-x |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Key words:PAF (platelet activating factor)-receptor - Keratinocytes - Fibroblasts - Immunohistochemistry - Western-blot |
K10plus-PPN: | 1807478203 |
Verknüpfungen: | → Zeitschrift |
PAF-Receptor in inflammatory versus non inflammatory human epidermis, cell cultures and embryonal cells / Bayerl, Christiane [VerfasserIn]; June 2003 (Online-Ressource)
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