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Status: Bibliographieeintrag

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Verfasst von:Sturm, Jörg [VerfasserIn]   i
 Keese, Michael [VerfasserIn]   i
 Petruch, Beate [VerfasserIn]   i
 Bönninghoff, Roderich [VerfasserIn]   i
 Zhang, Honyue [VerfasserIn]   i
 Gretz, Norbert [VerfasserIn]   i
 Hafner, Mattias [VerfasserIn]   i
 Post, Stefan [VerfasserIn]   i
 Mc Cuskey, Robert S. [VerfasserIn]   i
Titel:Enhanced green fluorescent protein-transfection of murine colon carcinoma cells
Titelzusatz:key for early tumor detection and quantification
Verf.angabe:Jörg W. Sturm, Michael A. Keese, Beate Petruch, Roderich G. Bönninghoff, Hongyue Zhang, Norbert Gretz, Mattias Hafner, Stefan Post & Robert S. Mc Cuskey
E-Jahr:2003
Jahr:September 2003
Umfang:11 S.
Illustrationen:Illustrationen
Fussnoten:Gesehen am 22.06.2022
Titel Quelle:Enthalten in: Clinical & experimental metastasis
Ort Quelle:Dordrecht : Springer Science + Business Media B.V., 1983
Jahr Quelle:2003
Band/Heft Quelle:20(2003), 5 vom: Sept., Seite 395-405
ISSN Quelle:1573-7276
Abstract:Many animal models for metastatic colorectal cancer represent clinical manifestations just inaccurately. We introduce a novel mouse model for metastastatic colorectal cancer. In order to remain close to the clinical disease a syngenic murine colon carcinoma cell line (colon 26 cells) was transfected with enhanced green fluorescent protein (EGFP). The transfected cells maintain the highly malignant attributes of the wild-type cells. Following injection into the portal circulation of Balb/c-mice, liver metastases occur in the same time span. Using the fluorescent attributes of the transfected cells, an approximation of the tumor load in liver tissue can be achieved by fluorescence activated cell sorting (FACS) and fluoroscan analysis. Tumor cell load in liver tissue can be accurately measured by Northern blot and Western blot analysis of liver tissue containing EGFP-transfected colon cancer metastases (1250 cells/mg liver tissue and 1000 cells/mg liver tissue) respectively. Confocal microscopy and intravital microscopy confirmed the growth of tumor metastases, originating from the intravascular compartments. The presented animal model using EGFP-transfected colon 26 cells allows the detecting of tumor growth in vivo and post mortem, as well as an accurate quantification of the tumor load in the liver tissue.
DOI:doi:10.1023/A:1025470312074
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1023/A:1025470312074
 DOI: https://doi.org/10.1023/A:1025470312074
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:animal model
 colorectal
 confocal microscopy
 EGFP
 metastatic cancer
 syngenic
 tumor quantification
K10plus-PPN:1807480623
Verknüpfungen:→ Zeitschrift

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