Urokinase plasminogen activator receptor (CD87) expression of tumor-associated macrophages in ductal carcinoma in situ, breast cancer, and resident macrophages of normal breast tissue

• Verfasst von: Hildenbrand, Ralf [VerfasserIn]
• Verfasst von: Wolf, Georg [VerfasserIn]
• Verfasst von: Böhme, Beatrix [VerfasserIn]
• Verfasst von: Bleyl, Uwe [VerfasserIn]
• Verfasst von: Steinborn, Andrea [VerfasserIn]
• Titel: Urokinase plasminogen activator receptor (CD87) expression of tumor-associated macrophages in ductal carcinoma in situ, breast cancer, and resident macrophages of normal breast tissue
• Verf.angabe: Ralf Hildenbrand, Georg Wolf, Beatrix Böhme, Uwe Bleyl, and Andrea Steinborn
• E-Jahr: 1999
• Jahr: 01 July 1999
• Umfang: 10 S.
• Fussnoten: Gesehen am 23.06.2022
• Titel Quelle: Enthalten in: Journal of leukocyte biology
• Ort Quelle: Kettering : Oxford University Press, 1984
• Jahr Quelle: 1999
• Band/Heft Quelle: 66(1999), 1, Seite 40-49
• ISSN Quelle: 1938-3673
• DOI: doi:10.1002/jlb.66.1.40
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: blood monocytes
• Sach-SW: inflammation
• Sach-SW: urokinase receptor
• K10plus-PPN: 1807531252

Abstract

Macrophages concentrate urokinase-type plasminogen activator (uPA) at the cell surface by expressing urokinase receptors (uPAR) in order to focus the pericellular space plasminogen-dependent proteolysis important in matrix remodeling and cell movement. This study examines the uPAR levels of tumor-associated macrophages (TAM) of invasive breast carcinomas, of TAMs from ductal carcinoma in situ (DCIS) and of macrophages derived from normal (non-tumor) breast tissue. TAMs from invasive breast carcinomas (n = 30), from DCIS (n = 12), and macrophages from normal breast tissue (n = 30) were cultured and immunocytochemically phenotyped by using a panel of antibodies. Urokinase receptor levels were determined by Western blot analysis and in cell-free supernatants by enzyme-linked immunosorbent assay. Urokinase receptor cell surface fluorescence intensity was determined by FACS and by confocal laser scan microscopy. Urokinase-receptor mRNA was detected by in situ hybridization. TAMs of invasive breast carcinomas and of DCIS possess significantly elevated uPAR levels compared with macrophages derived from normal breast tissue. Conclusions: activated macrophages with elevated uPAR levels belong to inflammatory areas in close vicinity of infiltrating and non-infiltrating (DCIS) tumor cells. Blood monocytes that possess elevated uPAR-levels may be selectively recruited from the bloodstream to inflammatory sites close to carcinoma cells, and/or breast cancer and precursor lesions may induce elevated uPAR-levels in TAMs by paracrine interactions. J. Leukoc. Biol. 66: 40-49; 1999.