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Status: Bibliographieeintrag

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Verfasst von:Endlich, Nicole [VerfasserIn]   i
 Sunohara, Masataka [VerfasserIn]   i
 Nietfeld, Wilfried [VerfasserIn]   i
 Wolski, Eryk W. [VerfasserIn]   i
 Schiwek, Daniel [VerfasserIn]   i
 Kränzlin, Bettina [VerfasserIn]   i
 Gretz, Norbert [VerfasserIn]   i
 Kriz, Wilhelm [VerfasserIn]   i
 Eickhoff, Holger [VerfasserIn]   i
 Endlich, Karlhans [VerfasserIn]   i
Titel:Analysis of differential gene expression in stretched podocytes
Titelzusatz:osteopontin enhances adaptation of podocytes to mechanical stress
Verf.angabe:Nicole Endlich, Masataka Sunohara, Wilfried Nietfeld, Eryk W. Wolski, Daniel Schiwek, Bettina KräNzlin, Norbert Gretz, Wilhelm Kriz, Holger Eickhoff, Karlhans Endlich
E-Jahr:2002
Jahr:19 September 2002
Umfang:24 S.
Illustrationen:Illustrationen
Fussnoten:Gesehen am 24.06.2022
Titel Quelle:Enthalten in: Federation of American Societies for Experimental BiologyThe FASEB journal
Ort Quelle:Hoboken, NJ : Wiley, 1987
Jahr Quelle:2002
Band/Heft Quelle:16(2002), 13 vom: Sept., Seite 1-24
ISSN Quelle:1530-6860
Abstract:Glomerular hypertension is a major determinant advancing progression to end-stage renal failure. Podocytes, which are thought to counteract pressure-mediated capillary expansion, are increasingly challenged in glomerular hypertension. Studies in animal models of glomerular hypertension indicate that glomerulosclerosis develops from adhesions of the glomerular tuft to Bowman's capsule due to progressive podocyte loss. However, the molecular alterations of podocytes in glomerular hypertension are unknown. In this study, we determined the changes in gene expression in podocytes induced by mechanical stress in vitro (cyclic biaxial stretch, 0.5 Hz, 5% linear strain, 3 days) using cDNA arrays (6144 clones). Sixteen differentially regulated genes were identified, suggesting alterations of cell-matrix interaction, mitochondrial/metabolic function, and protein synthesis/degradation in stretched podocytes. The transcript for the matricellular protein osteopontin (OPN) was most strongly up-regulated by stretch (approximately threefold). By reverse transcriptase-polymer chain reaction, up-regulation of OPN mRNA was also detected in glomeruli of rats treated for 2.5 wk with desoxycorticosterone acetate-salt, an animal model of glomerular hypertension. In cultured podocytes, OPN coating induced a motile phenotype increasing actin nucleation proteins at cell margins and reducing stress fibers and focal adhesions. Intriguingly, additional OPN coating of collagen IV-coated membranes accelerated stretch-induced actin reorganization and markedly diminished podocyte loss at higher strain. This study delineates the molecular response of podocytes to mechanical stress and identifies OPN as a stretch-adapting molecule in podocytes.
DOI:doi:10.1096/fj.02-0125fje
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1096/fj.02-0125fje
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1096/fj.02-0125fje
 DOI: https://doi.org/10.1096/fj.02-0125fje
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:actin cytoskeleton
 cDNA array
 DOCA-salt
 Glomerular hypertension
 nephron degeneration
K10plus-PPN:1807765350
Verknüpfungen:→ Zeitschrift

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