Does metformin influence the insulin-, IGF I- and IGF II-receptor gene expression and Akt phosphorylation in human decidualized endometrial stromal cells?

• Verfasst von: Capp, Edison [VerfasserIn]
• Verfasst von: Jauckus, Julia [VerfasserIn]
• Verfasst von: von Eye Corleta, Helena [VerfasserIn]
• Verfasst von: Toth, Bettina [VerfasserIn]
• Verfasst von: Strowitzki, Thomas [VerfasserIn]
• Verfasst von: Germeyer, Ariane [VerfasserIn]
• Titel: Does metformin influence the insulin-, IGF I- and IGF II-receptor gene expression and Akt phosphorylation in human decidualized endometrial stromal cells?
• Verf.angabe: Edison Capp, Julia Jauckus, Helena von Eye Corleta, Bettina Toth, Thomas Strowitzki, Ariane Germeyer
• E-Jahr: 2011
• Jahr: 12 June 2011
• Umfang: 6 S.
• Fussnoten: Gesehen am 27.06.2022
• Titel Quelle: Enthalten in: European journal of obstetrics & gynecology and reproductive biology
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1971
• Jahr Quelle: 2011
• Band/Heft Quelle: 158(2011), 2, Seite 248-253
• ISSN Quelle: 1872-7654
• DOI: doi:10.1016/j.ejogrb.2011.05.012
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Akt phosphorylation
• Sach-SW: Endometrium
• Sach-SW: IGF receptor
• Sach-SW: Insulin receptor
• Sach-SW: Metformin
• K10plus-PPN: 1807898377

Abstract

Objective - To assess the effects of metformin on insulin-, IGF I-, and IGF II-receptor gene expression and Akt phosphorylation in decidualized human endometrial stromal cells (ESC) after stimulation with insulin, IGF I and II. - Study design - ESC were isolated from healthy, regularly cycling women and after two passages decidualized with estrogen/progesterone±metformin. Cells were incubated with insulin, IGF I or IGF II for 1, 5, and 10min to assess Akt phosphorylation by Western blot. To investigate the insulin-, IGF I- and IGF II-receptor gene expression ESC were incubated with insulin, IGF I or IGF II for 6 and 24h. - Results - Insulin- and IGF I-receptor gene expression in ESC changed significantly after incubation with insulin, IGF I or IGF II. This was further augmented in metformin pretreated cells, while IGF II-receptor gene expression changed particularly after pretreatment with metformin. Akt phosphorylation peaked after 5min insulin, IGF I and IGF II stimulation in ESC in both control (control 0.08±0.03 vs. insulin 0.74±0.19, IGF I 0.68±0.22, IGF II 0.53±0.13, p<0.05) and metformin pretreated cells (control 0.03±0.01 vs. insulin 0.75±0.11, IGF I 0.74±0.15, IGF II 0.67±0.09, p<0.005). However, there was no significant difference between the control and metformin pretreated group. - Conclusion - Insulin, IGF I and IGF II lead to changes in their receptor gene expression and induced Akt phosphorylation in ESC. These effects were further highlighted in the presence of metformin.