Online-Ressource | |
Verfasst von: | Caudron-Herger, Maïwen [VerfasserIn] |
Müller-Ott, Katharina [VerfasserIn] | |
Mallm, Jan-Philipp [VerfasserIn] | |
Marth, Caroline [VerfasserIn] | |
Schmidt, Ute [VerfasserIn] | |
Fejes-Tóth, Katalin [VerfasserIn] | |
Rippe, Karsten [VerfasserIn] | |
Titel: | Coding RNAs with a non-coding function |
Titelzusatz: | Maintenance of open chromatin structure |
Verf.angabe: | Maïwen Caudron-Herger, Katharina Müller-Ott, Jan-Philipp Mallm, Caroline Marth, Ute Schmidt, Katalin Fejes-Tóth and Karsten Rippe |
E-Jahr: | 2011 |
Jahr: | 25 Oct 2011 |
Umfang: | 15 S. |
Fussnoten: | Gesehen am 28.06.2022 |
Titel Quelle: | Enthalten in: Nucleus |
Ort Quelle: | Austin, Tex. : Landes Bioscience, 2010 |
Jahr Quelle: | 2011 |
Band/Heft Quelle: | 2(2011), 5, Seite 410-424 |
ISSN Quelle: | 1949-1042 |
Abstract: | The multi-layered organization of the genome in a large nucleoprotein complex termed chromatin regulates nuclear functions by establishing subcompartments with distinct DNA-associated activities. Here, we demonstrate that RNA plays an important role in maintaining a decondensed and biologically active interphase chromatin conformation in human and mouse cell lines. As shown by RNase A microinjection and fluorescence microscopy imaging, digestion of single-stranded RNAs induced a distinct micrometer scale chromatin aggregation of these decondensed regions. In contrast, pericentric heterochromatin was more resistant to RNase A treatment. We identified a class of coding RNA transcripts that are responsible for this activity, and thus termed these ‘chromatin-interlinking’ RNAs or ciRNAs. The initial chromatin distribution could be restored after RNase A treatment with a purified nuclear RNA fraction that was analyzed by high-throughput sequencing. It comprised long >500 nucleotides (nt) RNA polymerase II (RNAP II) transcripts that were spliced, depleted of polyadenylation and was enriched with long 3'-untranslated regions (3’-UTRs) above 800 nt in length. Furthermore, similar reversible changes of the chromatin conformation and the RNAP II distribution were induced by either RNA depletion or RNAP II inhibition. Based on these results we propose that ciRNAs could act as genome organizing architectural factors of actively transcribed chromatin compartments. |
DOI: | doi:10.4161/nucl.2.5.17736 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt. Volltext: https://doi.org/10.4161/nucl.2.5.17736 |
DOI: https://doi.org/10.4161/nucl.2.5.17736 | |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1808043030 |
Verknüpfungen: | → Zeitschrift |