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Status: Bibliographieeintrag

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Verfasst von:Beck, Grietje [VerfasserIn]   i
 Hermes, Wilhelm-Clothar [VerfasserIn]   i
 Yard, Benito A. [VerfasserIn]   i
 Kaszkin, Marietta [VerfasserIn]   i
 Zabern, Detlef von [VerfasserIn]   i
 Schulte, Jutta [VerfasserIn]   i
 Haak, Markus [VerfasserIn]   i
 Prem, Katharina [VerfasserIn]   i
 Krimsky, W. [VerfasserIn]   i
 Ackern, Klaus van [VerfasserIn]   i
 Woude, Fokko J. van der [VerfasserIn]   i
 Yedgar, Saul [VerfasserIn]   i
Titel:Amelioration of endotoxin-induced sepsis in rats by membrane anchored lipid conjugates
Verf.angabe:Grietje Ch Beck, Wilhelm C. Hermes, Benito A. Yard, Marietta Kaszkin, Detlef von Zabern, Jutta Schulte, Markus Haak, Katharina Prem, W. Krimsky, Klaus van Ackern, Fokko J. van der Woude, Saul Yedgar
Jahr:2003
Umfang:7 S.
Fussnoten:Gesehen am 28.06.2022
Titel Quelle:Enthalten in: Critical care medicine
Ort Quelle:Hagerstown, Md. : Lippincott Williams & Wilkins, 1973
Jahr Quelle:2003
Band/Heft Quelle:31(2003), 7, Seite 2015-2021
ISSN Quelle:1530-0293
Abstract:OBJECTIVE: In the pathogenesis of septic shock, caused by either bacterial toxins or trauma, increased production of multiple proinflammatory mediators, such as phospholipase A(2) (PLA(2)), cytokines, and chemokines, is known to be of major importance. The present study was undertaken to investigate the influence of a newly designed extracellular PLA(2) inhibitor (ExPLI) on synthesis of proinflammatory mediators and mortality rate in a rat sepsis model. DESIGN: Prospective, randomized animal study. SETTING: Experimental laboratory. SUBJECTS: Male Wistar-rats weighing 200-300 g. INTERVENTIONS: Mortality was induced by intraperitoneal bolus administration of lipopolysaccharide 15 mg/kg in 22 rats that were pretreated with NaCl or ExPLI (150 mg/kg). Furthermore, nine rats received a sublethal bolus of lipopolysaccharide (7.5 mg/kg) and nine rats received lipotechoic acid (8 mg/kg) simultaneously with or after ExPLI administration. Blood samples were collected from these rats, and cytokine concentrations were assessed by enzyme-linked immunosorbent assay. Lung and kidney were removed for RNA isolation and immunohistological analysis. MEASUREMENTS AND MAIN RESULTS: ExPLI treatment significantly reduced lipopolysaccharide-induced mortality of rats (90.9 and 36.4%, p <.05). Up-regulation of tumor necrosis factor-alpha and interleukin-6 production in serum after endotoxin treatment was significantly inhibited when ExPLIs were administered at the time of or before sepsis induction by using lipopolysaccharide or lipotechoic acid (p <.01). Similarly, messenger RNA expression of secreted PLA(2)-IIA, interleukin-1, or inducible nitric oxide synthase and the expression of intercellular adhesion molecule-1 were significantly down-regulated in lung and kidney of ExPLI-treated rats, as demonstrated by RNase protection assay, reverse transcription-polymerase chain reaction, or immunohistochemistry. CONCLUSIONS: ExPLIs may be considered as potentially effective compounds to prevent the production of inflammatory mediators and to improve mortality rate in septic patients.
DOI:doi:10.1097/01.CCM.0000074717.46748.64
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1097/01.CCM.0000074717.46748.64
 Volltext: https://journals.lww.com/ccmjournal/Fulltext/2003/07000/Amelioration_of_endotoxin_induced_sepsis_in_rats.20.aspx
 DOI: https://doi.org/10.1097/01.CCM.0000074717.46748.64
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Animals
 Blood Proteins
 Cytokines
 Drug Carriers
 Inflammation Mediators
 Intercellular Adhesion Molecule-1
 Kidney
 Lipopolysaccharides
 Lung
 Male
 Membrane Lipids
 Phospholipases A
 Rats
 Rats, Wistar
 Shock, Septic
K10plus-PPN:1808384342
Verknüpfungen:→ Zeitschrift

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